Luminex Molecular Diagnostics, Inc.

Frank Merante

Susan Bortolin

Daniel Fieldhouse

Daniel Kobler

Roman L Zastawny

Richard A Janeczko

Margot Boszko

Barbara Galvan-Goldman

Hemanshu Modi

James D Gordon

The methods and compositions of the present invention allow for the evaluation of a nucleotide expansion, contraction or deletion. In one embodiment, the present invention provides a method for detecting a nucleotide expansion, contraction or deletion comprising amplifying a target nucleic acid sequence with a primer pair, each primer comprising a target specific sequence and a differentiating tag sequence, labeling the target nucleic acid sequence to produce a dual-tagged amplification product; digesting the dual-tagged amplification product to produce an expansion region fragment and an internal control fragment; hybridizing the fragments to separate capture complexes; detecting the signals produced by the labels on the immobilized fragments and comparing the intensity of the signals to detect the nucleotide expansion, contraction or deletion region.

Use of dual-tags for the evaluation of genomic variable repeat regions

The present invention provides a method for the simultaneous identification of two or more single base changes in a plurality of target nucleotide sequences that are markers associated with cardiovascular diseases such as deep vein thrombosis and the like. Multiplex detection is accomplished using multiplexed tagged allele specific primer extension (ASPE) and hybridization of such extended primers to a probe, preferably an addressable anti-tagged support.

Method of detecting mutations associated with thrombosis

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Etobicoke, Canada

Frank Merante