Norchip A/s

Nye Colifast As

Pretect As

Frank Karlsen

Klaus Stefan Drese

Rainer Gransee

Tobias Baier

Lars A Solli

Anja Gulliksen

Thomas Hansen-Hagge

Liv Furuberg

Einar Morland

Geir Morland

Bente Falang

F Inst Für Mikrotechnik Mainz Gmbh Von Germar

Jan Inst Of Microtechnology Lichtenberg

Sabeth Inst Of Microtechnology Verpoorte

F Inst Für Mikrotechnik Mainz Gmbh Schonfeld

An in vitro method is provided for screening human female subjects to assess their risk of developing cervical carcinoma which comprises screening the subject for expression of mRNA transcripts from the E6 and optionally the L1 gene of human papillomavirus, wherein subjects positive for expression of L1 and/or E6 mRNA are scored as being at risk of developing cervical carcinoma. Kits for carrying out such methods are also provided.

Method for detecting human papillomavirus mRNA

The present invention provides an integrated lab-on-a-chip device for carrying out a nucleic acid extraction process on a fluid sample containing cells and/or particles, the device comprising: (a) a sample inlet () for loading of a fluid sample, (b) a lysis unit () for lysis of cells and/or particles present in the fluid sample, (c) a reservoir of lysis fluid () for the lysis unit, (d) a nucleic acid extraction unit () downstream of the lysis unit, and (e) reservoirs of first washing buffer and eluant fluid () for the nucleic acid extraction unit, wherein the device further comprises (f) a mixing unit () downstream of the nucleic acid extraction unit, and (g) a source of mixing fluid () for the mixing unit. The reservoirs of lysis fluid, first washing buffer and eluant fluid may be provided parallel to one anther so that they may be actuated by a single pump.

Device for carrying out cell lysis and nucleic acid extraction

There is disclosed a sensitive method for specific detection of ligands comprising the steps of (a) contacting the sample with reagents capable of forming a reagent complex, which reagent complex comprises a receptor capable of specifically binding to said ligand and a nucleic acid molecule; and (b) detecting any complexes formed by binding of the receptor part of said reagent complex to ligand present in the sample by specifically detecting the presence of the nucleic acid molecule by amplifying a region of the nucleic acid and simultaneously detecting products of the amplification reaction in real-time.

Ligand detection method

The present invention relates to a microfabricated reaction chamber system and a method of fluid transport. The system may be used, for example, in a method of carrying out a nucleic acid sequence amplification and detection process on a nucleic acid sample. The microfabricated chamber system comprises an inlet port and/or an outlet port and a variable volume chamber in fluid communication with said port(s), wherein altering the volume of the variable volume chamber effects and/or restricts flow of a fluid sample to and/or from said port(s).

Fluid manipulation in a microfabricated reaction chamber systems

A method for the identification of the presence of and and/or in liquid or liquified samples. The method employs novel oligonucleotide primers based on sequences of the LamB gene and the transposase gene Tn1546. The method involves enrichment of the liquid or liquified sample, preferably using a selective medium, obtaining DNA from bacteria in the sample, and PCR amplification of the DNA using the novel oligonucleotide primers. Kits with the primers are also contemplated.

Method for identification of the indicators of contamination in liquid samples

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Klokkarstua, Norway

Frank Karlsen