Kuros Biosciences Ag

Checkmate Pharmaceuticals, Inc.

Cytos Biotechnology Ag

Frank Hennecke

Marcel Emmerling

Holger Pfründer

Martin Rhiel

Philipp Steiner

Evan David Walters

Isabelle Lacan

Chi Lan Le

Holger Pfruender

Philippe Saudan

Jennifer Erickson

The present invention relates to processes for producing compositions comprising (i) a virus-like particle of an RNA bacteriophage, and (ii) aggregated oligonucleotides, wherein said aggregated oligonucleotides are packaged into said virus-like particle. The invention further provides processes for producing nucleotide compositions comprising aggregated oligonucleotides suitable for use in the aforementioned processes before. Moreover, the invention further provides nucleotide compositions comprising aggregated oligonucleotides. Furthermore, the invention further provides compositions comprising (i) a virus-like particle of an RNA bacteriophage, and (ii) aggregated oligonucleotides, wherein said aggregated oligonucleotides are packaged into said virus-like particle.

Packaging oligonucleotides into virus-like particles

This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

Scalable fermentation process

Growing community of inventors

Dietlikon, Switzerland

Frank Hennecke