Dow Agrosciences LLC

Agrigenetics, Inc.

Corteva Agriscience LLC

Frank Burroughs

Jayakumar Pon Samuel

Narasimha Chary Samboju

Steven Robert Webb

Kerrm Y Yau

Paul R Schmitzer

William M Ainley

Gaofeng Lin

Mark W Zettler

Jeffrey Beringer

Robbi J Garrison

Suraj K Dixit

P Samuel Jayakumar

Yang Yang

Kristen Curlee

Pon Samuel Jayakumar

Jeffrey R Beringer

William Michael Ainley

Robbi Garisson

An apparatus and a method of using at least one of a distance sensor and a light intensity sensor to adjust the distance between an artificial light source and plant canopy to control light intensity at the plant canopy is disclosed.

Lighting system

Provided are methods for introducing a molecule of interest into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Methods for transferring molecular substances into plant cells

Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using dendrimers, and optionally one or more Cell Penetrating Peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Use of dendrimer nanotechnology for delivery of biomolecules into plant cells

Methods for introducing a linear nucleic acid molecule of interest into a cell comprising a cell wall include use of nanoparticles coated with polyethylene glycol. In some embodiments, the cell comprising a cell wall is a plant cell. Methods include genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with linear nucleic acid molecules.

Linear DNA molecule delivery using PEGylated quantum dots for stable transformation in plants

Provided are methods for introducing a molecule of interest into a plant cell having a cell wall by using a QD-peptide conjugate having a quantum dot (QD) with one or more cell penetrating peptides (CPPs). Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.

Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants

A method of introducing a molecule of interest into a plant cell having a cell wall includes interacting a gamma-zein peptide with a molecule of interest to form a gamma-zein linked structure. The gamma-zein linked structure is then placed in contact with the plant cell having a cell wall, and allowing uptake of the gamma-zein linked structure into the plant cell. Alternatively, a gene of interest can be expressed in a plant cell having an intact cell wall by interacting a gamma-zein peptide with the gene of interest to form a gamma-zein linked gene structure, allowing uptake of the gamma-zein linked gene structure into the plant cell, and expressing the gene of interest in the plant cell and its progeny.

Plant peptide gamma-zein for delivery of biomolecules into plant cells

Methods for introducing a functionalized linear nucleic acid cassette molecule of interest into a plant cell comprising a cell wall include use of nanoparticles. In some embodiments, the cell comprising a cell wall is a cultured plant cell. Methods include genetically or otherwise modifying plant cells and for treating or preventing disease in any plant, especially crop plants. Transgenic plants include a nucleic acid molecule of interest produced by regeneration of whole plants from plant cells transformed with functionalized linear nucleic acid cassette molecules.

Production of functionalized linear DNA cassette and quantum dot/nanoparticle mediated delivery in plants

The subject invention provides simple and consistent methods to break suspension cell aggregates to single cells with intact primary cell walls. The subject invention relates in part to cell separation of suspension cell aggregates cultured in medium containing pectin-degrading enzymes or tubulin de-polymerizing compounds including colchicine. The subject invention also relates to novel uses of compounds for such purposes. Another aspect of the subject invention relates to transformation of the subject, isolated cells. Such processes simplify and integrate single-cell-based transformation and selection processes into transgenic and transplastomic event-generation work processes. The subject invention also removes technical constraints and produces marker-free and uniformly expressing transgenic lines in a high throughput fashion to support various needs of animal health, biopharma, and trait and crop protection platforms.

Growing community of inventors

Noblesville, IN, United States of America

Frank Burroughs