Roche Molecular Systems, Inc.

Roche Diagnostics Operations, Inc.

Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

Dako A/s

Hoffmann-La Roche Inc.

F. Hoffmann-La Roche Ag

Epigenomics Ag

Frank Bergmann

Dieter Heindl

Herbert Von Der Eltz

Christoph Seidel

Frank Seela

Hans-Peter Josel

Dorothea Sizmann

Victor I Lyamichev

Michael Schraeml

Jigar Patel

Thomas J Albert

Hannes Kuchelmeister

Wojtek Steffen

Thomas Fischer

Luisa De Cola

Peter Crisalli

Walter Eberle

Sebastian Dziadek

Robert Cysewski

Jesus Miguel Fernandez Hernandez

Thomas Streidl

Sebastian Johannes Pomplun

Rupert Herrmann

Meng Taing

Dmitriy Gremyachinskiy

Bruno Frey

Andrew Trans

Christine Markert-Hahn

Kurt Weindel

Yann A Astier

Emad Sarofim

Uwe Kobold

Troels Koch

Dirk Block

Piet Herdewijn

Aruna Ayer

Tobias Oelschlaegel

Helmut Rosemeyer

Mara Boenitz-Dulat

Thomas Walter

Juraj Topolancik

Christian Birkner

Manfred Watzele

Ralf Zielenski

Stefanie Froehner

Martin Schatte

Bernd Buchberger

Dirk Kessler

Simon Ferdinand Loibl

Joerg Kleiber

Christian Weilke

Reinhard Beck

Reiner Babiel

Horst Donner

Heike Zitzer

Enno Schweinberger

Omid Khakshoor

Mohamed Yosry Hassan Mohamed

Georg Becher

Nina Lassonczyk

Lars Hillringhaus

Elena Longhi

Gunter Lampert

Thomas Albert

Rainer Beckert

Johannes Stoeckel

Thomas Welzel

Christian Wellner

Ian Quang Chau

Sara Kelley

Marcus Schmid

Hannah Marie Kallewaard-Lum

Nikolaus Klaus-Peter Stengele

Hasham Saqib

Harald Debelak

Helmut Rosenmeyer

Michael Kirchgesser

Joerg Kleibler

Stephanie Froehner

Jessica Steger-Domandl

Dieter Hiendl

Ralf Mauritz

Rita Härteis

Christine Market-Hahn

Dorothea Sitzmann

Steffen Wojtek

Rongxin Nie

Yann Astier

Michael Schaeml

Seong-Ho Shin

Nikolaus-Peter Stengele

The present disclosure relates to the use of a capping and capture reagent in solid phase peptide synthesis. The present disclosure further relates to a method of solid phase peptide synthesis, wherein a capping and capture reagent according to the present disclosure is used. The present disclosure further relates to a method for purification of a (full-length) synthetic peptide via use of a capping and capture reagent according to the present disclosure. The present disclosure also relates to a kit comprising a capping and capture reagent according to the present disclosure and an amino oxy resin or a hydrazine resin and the use of the kit.

HPLC free purification of peptides by the use of new capping and capture reagents

The disclosure relates to tagged nucleoside compounds comprising a nucleotide polyphosphate covalently attached to a tag, wherein the compound is a polymerase substrate and the polymer moiety is capable of entering a nanopore linked to the polymerase and thereby altering the flow of ions through the nanopore. The disclosure also provides methods for preparing the tagged nucleoside compounds and for their use as tags in nanopore-based nucleic acid detection and sequencing.

Tagged nucleoside compounds useful for nanopore detection

The present disclosure provides 3' protected nucleotides, including those 3&#x2032; protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3&#x2032; protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.

3' protected nucleotides

The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods.

Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection

Molecules may be analyzed (e.g., sequencing of nucleic acid molecules) by tunneling recognition at a tunneling junction. Embodiments of the present invention may allow detecting individual nucleotides and the sequencing of a nucleic acid molecule using a tunneling junction. By labeling a specific nucleotide with a moiety, tunneling junctions may generate a signal with a suitable signal-to-noise ratio. The tunneling recognition uses a tunneling current that is mostly through the moiety rather than mostly through the nucleotide or a portion of the molecule of interest. Because a single nucleotide can be detected with a signal with a suitable signal-to-noise ratio resulting from the tunneling current passing through the moiety, embodiments of the present invention may allow for fast detection of nucleotides using a tunneling current.

Tunneling junctions for sequencing

The present invention provides a new building block for peptide synthesis, which introduces a cleavage site that can be used to generate cleavable fragments subsequent to a peptide sequence.

Cleavable linker for peptide synthesis

The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.

Set of oligonucleotide probes as well as methods and uses thereto

The present invention relates to improving the processing rate of a sequencing reaction, for example in a nanopore sequencing reaction, by means of using improved nucleoside-tags. The tags are linked to the nucleoside phosphate via a Pictet Spengler reaction. Exemplary sequencing reactions that are improved by the present methods include nanopore-based nucleic acid sequencing-by-synthesis reactions.

Method for labeling of aldehyde containing target molecules

In one aspect, the present disclosure provides a system and method for the identification and characterization of a transglutaminase. Further, the present disclosure provides transglutaminase enzymes for forming isopeptide bonds, methods of forming isopeptide bonds in the presence of transglutaminases, and substrate tags for use with transglutaminases. In another aspect, the present disclosure provides glutamine-containing substrates (or Q-tag substrates) that are more resistant to proteases/clipping and therefore, more stable, than other Q-tag substrates, and their uses in substrate tags for cross-linking to an amine-donor tag via an isopeptide bond mediated by a microbial transglutaminase.

System and method for identification and characterization of transglutaminase species

According to one aspect, the present disclosure provides a method of identifying a substrate of a transglutaminase using a peptide array comprising a plurality of peptides. The method includes the steps of contacting the peptides in the peptide array with the transglutaminase, allowing the transglutaminase to bind to the peptides, and identifying the substrate of the transglutaminase.

Identification of transglutaminase substrates and uses therefor

The present description relates to a method for binding to a target molecule having an aldehyde compound derived from N-(2-aminoethyl)pyrrole, which compound also has a moiety of interest, to compounds (conjugates) obtained by this method, having both the target molecule and the moiety of interest and to novel substances derived from N-(2-aminoethyl)pyrrole. In one embodiment, the compound has the formula of Formula II wherein R1, R2 and R3 independently are H, substituted or unsubstituted alkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, or substituted or unsubstituted heteroaryl, wherein R4, R5, R6, R7 and R8 independently are H, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted aryl, substituted or unsubstituted heteroaryl, or -LM, wherein L is absent or is a linker and M is a moiety of interest selected from a nucleotide, an oligonucleotide, a peptide, a label, a cytotoxic agent, a partner of a binding pair and a functional group, wherein two of R4, R5, R6, R7, and R8 optionally are linked to form a substituted or unsubstituted cycloalkyl or a substituted or unsubstituted heterocycloalkyl, and T is a target molecule selected from the group consisting of a solid phase, a polypeptide, a protein, a carbohydrate, a nucleotide and a nucleic acid, with the proviso that at least one of R4, R5, R6, R7 or R8 is -LM.

Herein is reported a method for the enzymatic production of a thioester comprising incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue), ii) a second polypeptide that has at its N-terminus a cysteine amino acid residue or is a cysteinyl compound, and iii) a third polypeptide with sortase A activity.

Production of thioesters using sortase

Growing community of inventors

Iffeldorf, Germany

Frank Bergmann