Metabolix, Inc.

Joule Unlimited Technologies, Inc.

Inari Agriculture Technology, Inc.

Yield10 Bioscience, Inc.

Joule Unlimited, Inc.

Tepha, Inc.

Flagship Pioneering Innovations V, Inc.

Joule Unilimited Technologies, Inc.

Cj Research Center LLC

Frank Anthony Skraly

David P Martin

Oliver P Peoples

Simon F Williams

Dan Eric Robertson

Christian Perry Ridley

David Arthur Berry

Noubar Boghos Afeyan

Martha Sholl

Gjalt W Huisman

Brian D Green

Nikos Basil Reppas

Sriram Kosuri

Kristi D Snell

Yajie Niu

Regina Wilpiszeski

Thomas Martin Ramseier

Meghna Malik

Erik William Clement

William R Farmer

Jacob Carter Harrison

Venkatesh Bollina

Ning Li

George M Church

Johan Van Walsem

John Patrick Casey, Jr

Erik Anderson

Christopher L Mirley

Randall William Shultz

Kevin A Sparks

John Licata

Jihong Tang

Yossef Shabtai

Madana M R Ambavaram

Jeffrey A Bickmeier

Brian Prescott Fiske

Kristine Yu

Nirmala Sharma

Dong-Eun Chang

Ann D'Ambruoso

Melarkode S Sivasubramanian

Michael Connor

Eric James Devroe

Yuanyuan Ji

Claire Burkitt

Kieran Ryan

Jeff Bickmeier

Chenfeng Lu

Thomas M Ramseier

Brian Green

Eric Devroe

A modified plant is provided. The modified plant includes a polynucleotide including a promoter operably linked to a coding sequence. The coding sequence encodes a transcription factor that has at least 30% sequence identity to one or more of SEQ ID NOs: 27, 204, 10, 19, 1-9, 11-18, 20-26, 28-203, 205, 237, or 242-310. The promoter is non-cognate with respect to the coding sequence. Also provided is a method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by transformation. Also provided is a method for producing a modified plant having increased seed yield and/or oil content in comparison to a reference plant by editing.

Modified plants comprising a polynucleotide comprising a non-cognate promoter operably linked to a coding sequence that encodes a transcription factor

A genetically engineered land plant that expresses a protein that has homology to a plant invertase inhibitor and/or a pectin methylesterase inhibitor and that increases seed yield with increased expression ('an ISY protein') is disclosed. The plant comprises a modified gene for the ISY protein. The ISY protein comprises one or more of an ISY protein ofcomprising SEQ ID NO: 2 or a fragment,homolog, or ortholog thereof. The modified gene comprises a promoter and a nucleic acid sequence encoding the ISY protein. The promoter is non-cognate with respect to the nucleic acid sequence encoding the ISY protein. The modified gene is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the ISY protein. A genetically engineered land plant that expresses an RNA that increases seed yield with increased expression also is disclosed.

Genetically engineered land plants that express an increased seed yield protein and/or an increased seed yield RNA

Disclosed herein are compositions and methods for effecting alterations at a defined location in the genome of a plant cell or plant protoplast. Further disclosed are methods using an RNA-guided nuclease to alter a target nucleotide sequence in a cell or protoplast of a plant; embodiments of such methods include treatments with chemical or physical reagents or treatment of the cell or protoplast with a specific thermal regime, such as a heat treatment.

Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA

A genetically engineered land plant that expresses a plant CCP1-like mitochondrial transporter protein is provided. The genetically engineered land plant comprises a modified gene for the plant CCP1-like mitochondrial transporter protein. The plant CCP1-like mitochondrial transporter protein is an ortholog of CCP1 ofof SEQ ID NO: 1 derived from a source land plant. The plant CCP1-like mitochondrial transporter protein is localized to mitochondria of the genetically engineered land plant based on a mitochondrial targeting signal intrinsic to the plant CCP1-like mitochondrial transporter protein. The modified gene comprises (i) a promoter and (ii) a nucleic acid sequence encoding the plant CCP1-like mitochondrial transporter protein. The promoter is non-cognate with respect to the nucleic acid sequence. The modified gene is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the plant CCP1-like mitochondrial transporter protein.

Genetically engineered land plants that express a plant CCP1-like mitochondrial transporter protein

Plant cell cultures as well as related methods, systems, and compositions for increasing the frequency and efficiency of plant genome editing are provided. Various plant cell growth conditions and/or treatments where such increases in gene editing frequencies are obtained are disclosed.

Methods for increasing gene-editing frequencies in maize cells

Plant cell culture

The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described.

Recombinant synthesis of medium chain-length alkanes

The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.

Engineered CO2 fixing microorganisms producing carbon-based products of interest

Recombinant hosts for producing polyhydroxyalkanoates and methods of producing polyhydroxyalkanoates from renewable carbon substrates are provided. Certain recombinant hosts that produce 5 carbon chemicals such as 5-aminopentanoate (5AP), 5-hydroxyvalerate (5HV), glutarate, and 1,5 pentanediol (PDO) are also provided. One embodiment provides a recombinant host expressing a gene encoding a heterologous enzyme selected from the group consisting of a polyhydroxyalkanoate synthase and a 5-hydroxyvalerate-CoA (5HV-CoA) transferase, wherein the host produces a polymer containing 5-hydroxyvalerate. Preferably, the host expresses both a polyhydroxyalkanoate synthase and a 5HV-CoA transferase. The host can be prokaryotic or eukaryotic. A preferred prokaryotic host is. The polymers produced by the recombinant hosts can be homopolymers or copolymers of 5-hydroxyvalerate. A preferred copolymer is poly(3-hydroxybutyrate-co-5-hydroxyvalerate).

Green process and compositions for producing poly(5HV) and 5 carbon chemicals

Processes and methods for making biobased gamma-butyrolactone from renewable carbon resources are described herein.

Process for gamma-butyrolactone production

The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert inorganic carbon and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.

Photoalkanogens with increased productivity

The invention provides methods and compositions for inhibiting proliferation of a modified host cell outside of a designated process condition. Compositions and methods for providing a host cell having reduced viability when exposed to natural conditions external to a controlled environment are disclosed.

Methods and compositions for limiting viability of a modified host cell outside of designated process conditions

The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.

Methods and compositions for the recombinant biosynthesis of fatty acids and esters

Recombinant processes are provided whereby additional genes are introduced intowhich have been genetically engineered to produce PHA so that the improved strains produce PHA homopolymers and copolymers directly from diols. In preferred embodiments, PHAs containing 4-hydroxybutyrate monomers are produced directly from 1,4-butanediol; PHAs containing 5-hydroxyvalerate are produced from 1,5-pentanediol; PHAs containing 6-hydroxyhexanoate (6HH) are produced from 1,6-hexanediol; PHAs containing 3-hydroxypropionate are produced from 1,3-propanediol; PHAs containing 2-hydroxypropionate (lactate) are produced from 1,2-propanediol (propylene glycol); PHAs containing 2-hydroxyethanoate (glycolate) are produced from 1,2-ethanediol (ethylene glycol). Genes encoding these same enzyme activities can be introduced or their expression amplified in wild type PHA producers to improve the production of PHA homopolymers and copolymers directly from diol and other alcohol feedstocks. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates.

Production of polyhydroxyalkanoates from polyols

Growing community of inventors

Watertown, MA, United States of America

Frank Anthony Skraly