The State University of New York

The Penn State Research Foundation

Quadrant Biosciences Inc.

Frank A Middleton

Steven D Hicks

Richard Uhlig

William Garrow Kerr

Sandra Fernandes Denney

Alexander Rajan

A method for evaluating or monitoring metabolic effects of exercise or injury by detecting and/or quantifying microR-NAs in saliva. Compositions and methods of treatment based on detection of microRNAs.

Method for monitoring metabolism before and after exercise or injury using salivary micro-RNAs

Provided are methods for detecting or diagnosing a traumatic brain injury or TBI by detecting concentration levels miRNAs associated with TBI in saliva. Methods for controlled and normalized comparisons of salivary miRNA concentration levels are further provided. Assay kits comprising salivary miRNAs, probes and/or primers for detecting salivary miRNAs are also provided.

Analysis and prediction of traumatic brain injury and concussion symptoms

This application provides methods to differentiate between subjects with autism spectrum disorder (ASD) and typically developing (TD) or developmentally delayed (DD) subjects using miRNA and/or microbiome levels detected in saliva samples and patient information. The method can be used to monitor the progress of ASD and guide its treatment. RNA-seq, qPCR, or other methods determine counts and abundance of miRNA or microbiomes. MicroRNA and/or microbiome sequencing data are refined by normalization to expression levels or abundance of time-invariant miRNAs and/or microbial RN As to control for time of sample collection or to compensate for circadian fluctuations in these levels. Multivariate logistic regression and nonlinear classification techniques are further used to select a panel of miRNAs and microbiomes that accurately differentiate between subjects with ASD, DD, and TD in subjects with an unknown ASD status. These panels of miRNAs and microbiomes may be developed into a RNA assay kit.

Analysis of autism spectrum disorder

The present invention relates to methods and kits for detecting a fusion mRNA transcript or polypeptide encoded by the fusion mRNA transcript. The method includes obtaining a biological sample from a subject and providing one or more reagents capable of binding a fusion mRNA transcript or polypeptide encoded by the fusion mRNA transcript, wherein the fusion mRNA transcript or polypeptide encoded by the fusion mRNA transcript comprises the 3' end of ex on 25 of INPP5D fused to the 5&#x2032; end of ex on 2 of ATG16L1 or the polypeptide encoded by the 3&#x2032; end of ex on 25 of INPP5D fused to the 5&#x2032; end of ex on 2 of ATG16L1, respectively.

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Fayetteville, NY, United States of America

Frank A Middleton