Commissariat a L'energie Atomique

Centre National De La Recherche Scientifique

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Azbil Corporation

Universitè De Montpellier

Yamatake Corporation

Université Joseph Fourier

Commissariat a L'energie

Françoise Vinet

Claude Vauchier

Patrick Chaton

Gilles Marchand

Daisuke Obara

Shinsuke Yamasaki

Yasuhiro Goshoo

Takaaki Kuroiwa

Antoine Hoang

Naohiro Ishikawa

Jean Dijon

Marc Delaunay

Nicolas Sarrut

Vincent Agache

Frederic Revol-Cavalier

Jean F Clerc

Florence Ricoul

Stéphane Getin

Pierre Barritault

Franck Martin

Olivier Constantin

Pascal Dumy

Michel Granier

Philippe Combette

Gérard Lanneau

Celine Escoffier

Eric Defrancq

Frédérique Mittler

The present invention relates to a method for functionalizing fluid lines () in a micromechanical device, the walls of which include an opaque layer. For this purpose, the invention provides a method for functionalizing a micromechanical device provided with a fluid line including a peripheral wall () having a surface () outside the line and an inner surface () defining a space () in which a fluid can circulate, the peripheral wall at least partially including a silicon layer (). The method includes the following steps: a) providing a device, the peripheral wall () of which at least partially includes a silicon layer () having, at least locally, a thickness (e) of more than 100 nm and less than 200 nm, advantageously of 160 to 180 nm; c) silanizing at least the inner surface of the fluid line; d) the localized, selective photo-deprotection on at least the inner surface of the silanized device by exposing the peripheral wall () at the point at which said wall has a thickness (e) of more than 100 nm and less than 200 nm, advantageously of 160 to 180 nm.

Method for functionalising fluid lines contained in a micromechanical device, micromechanical device including functionalised lines, and method for manufacturing same

The device of the invention comprises a support having a surface comprising an attachment zone (Z) capable of being functionalized with a probe (A) capable of binding, according to the pH and reversibly, to a target (B) so as to attach it; a working electrode (WE) and a counterelectrode (CE) for this working electrode, arranged on the support in the vicinity of the attachment zone; and means for applying a given electric current or a given potential to said working electrode so as to cause, when said attachment zone and said electrodes are immersed in an aqueous solution, a local variation in pH in the region of said attachment zone. The method for attaching and/or detaching a target or an object according to the invention uses this device, the attachment and/or the detachment being electrochemically controlled with the working electrode.

Device and methods for coupling/uncoupling a target or an object present in a sample

A microfluidic component comprises at least one channel () delineated by a top wall () and a bottom wall () and two opposite side walls (). The distance (P) between the top wall () and the bottom wall () of the channel () is greater than or equal to 25 micrometers and first and second sets of nanotubes () are respectively borne by the two opposite side walls () for the component to present a particularly high ratio between the contact surface and the available volume and a limited overall surface size. In addition, the distance between the two opposite side walls () is about a few micrometers and preferably comprised between 3 and 5 micrometers.

Microfluidic component with a channel filled with nanotubes and method for its production

A substrate for biochips is manufactured so that the substrate has a substrate surface having a reaction region capable of reacting with biological substances and a non-reaction region not reacting with the biological substances, sunken bottomed wells formed in the substrate surface, and a layer of a material capable of reacting with the biological substances having a surface exposed only at the bottoms of the bottomed wells, the exposed surface forming the reaction region.

Method for manufacturing substrate for biochip

A microreactor is shown having an inlet or feed channel, an inlet or feed zone for a flow of fluid, a reaction zone, an outlet zone and an outlet or evacuation channel, the zones and channels being in fluid communication, and at least one compound such as an enzyme capable of producing a biological or biochemical reaction with at least one constituent of the flow of fluid, the compound being attached to the surfaces of the inlet zone, reaction zone, and outlet zone.

Microreactor, process for the preparation thereof, and process for carrying out a biochemical or biological reaction

The invention relates to compounds comprising a photolabile protecting group and to the use thereof as coupling agents for the functionalisation of solid supports. The invention also relates to the solid supports functionalised by said compounds and to the use of same for the immobilisation of biological molecules of interest, such as nucleic acid molecules.

Coupling agents comprising a photolabile protecting group and uses thereof, such as for the functionalisation of solid supports

A substrate for biochips has a substrate surface having a reaction region capable of reacting with biological substances and a non-reaction region not reacting with the biological substances, sunken bottomed wells formed in the substrate surface, and a layer of a material capable of reacting with the biological substances having a surface exposed only at the bottoms of the bottomed wells, the exposed surface forming the reaction region.

Substrate for biochip

The invention concerns a device for enhancing fluorescence comprising a support () carrying fluorescence enhancement means (), the fluorescence enhancement means offering a reception surface for chemical or biological elements intended to be read by detection of a fluorescence signal emitted by a fluorophore, associated with the chemical or biological elements, under the effect of an excitation light beam. The fluorescence enhancement means () is made up of a thin, transparent, dielectric layer or a stack of thin, transparent, dielectric layers (to) ensuring a mirror function for the fluorescence signal and excitation light beam, the material of the thin layer or of each thin layer of the stack being chosen from among the following materials: TiO, TaO, HfO, ZrO, MgO, SiO, SiN, MgFand YF. The fluorescence enhancement device may be used for a biological or chemical optic sensor.

Device for enhancing broad band fluorescence with low loss and biological or chemical optic sensor using the same

The invention relates to &#x3c9;-silanyl n-alkanal compounds, method for production and use thereof in the fuctionalization of solid supports, solid supports functionalized by the compounds and the use of solid supports thus functionalized for the immobilization and/or synthesis of biological molecules of interest.

Silanyl-n-alkanal compounds, method for production and use thereof

The invention relates to a process for synthesizing nanorods of a carbide of one metal Mon a substrate, which comprises: The invention also relates to a process for growing nanorods of a carbide of one metal Mon a substrate from nanocrystals of this metal, to the substrates thus obtained and to their applications: fabrication of Microsystems provided with chemical or biological functionalities, in particular the fabrication of biosensors; electron emission sources, for example for flat television or computer screens; etc.

Method of synthesising and growing nanorods from a metal carbide on a substrate, substrates thus obtained and applications thereof

A process for characterizing, quantifying or mapping a nucleic acid or a nitrogenous base fixed on a support by using a photothermal (mirage effect) method. An improved process for manufacturing a nucleic acid biochip on which at least one nucleic acid synthesized in situ is fixed. A device for implementing these processes.

Method and device for analyzing nucleic acids immobilized on a support

The invention relates to novel compounds usable as liquid crystals in liquid crystal display devices utilizing the electrically controlled birefringence effect. These compounds comply with formula: ##STR1## in which R.sup.1 represents an alkoxy or alkyl radical with 1 to 12 carbon atoms and Z represents a single bond or a radical chosen from among: ##STR2## provided that R.sup.1 represents an alkyl radical when Z represents a single bond and R.sup.2 represents an alkyl radical with 1 to 12 carbon atoms. They can be obtained by reacting an acid chloride of formula R.sup.1 --z--COCl with a 2,2'-difluoro-4-alkoxy-4'-hydroxydiphenyl of formula: ##STR3##

2,2'-difluoro-4-alkoxy-4'-hydroxydiphenyls and their derivatives, their

The invention relates to novel compounds usable as liquid crystals in liquid crystal display devices utilizing the electrically controlled birefringence effect. These compounds comply with formula: ##STR1## in which R.sup.1 represents an alkoxy or alkyl radical with 1 to 12 carbon atoms and Z represents a single bond or a radical chosen from among: ##STR2## provided that R.sup.1 represents an alkyl radical when Z represents a single bond and R.sup.2 represents an alkyl radical with 1 to 12 carbon atoms. They can be obtained by reacting an acid chloride of formula R.sup.1 --z--COCL with a 2,2'-difluoro-4-alkoxy-4'-hydroxydiphenyl of formula: ##STR3##

The invention relates to a mixture comprising a nematic liquid crystal and a non-mesomorphous compound. The non-mesomorphous compound makes it possible to improve the elastic behavior of the nematic liquid crystal by increasing the value of the ratio K.sub.33 /K.sub.11, in which K.sub.33 represents the elastic bending constant and K.sub.11 the elastic fanning constant of the liquid crystal. The non-mesomorphous compound can be in accordance with the formula: ##STR1## in which R.sup.1 and R.sup.2, which can be the same or different, represent a hydrogen atom or an alkyl radical having 1 to 12 carbon atoms and n is an integer between 2 and 16 and can be associated with liquid crystals or liquid crystal mixtures containing a 1-(alkylcyclohexyl)-2-(alkylfluorobiphenylyl)-ethane. These mixtures can be used as a liquid crystal in a display device.

Mixtures comprising a nematic liquid crystal and a non-mesomorphic

The invention relates to a process for positioning the liquid medium in a liquid medium cell and to a corresponding cell. The cell comprises a liquid medium placed between two walls kept spaced from one another, e.g. by shims, and joined along the edge thereof, wherein it comprises first means for producing a first surface tension force in a first area and second means for producing a second surface tension force in a second area, the first surface tension force being higher the the second surface tension force, said means serving to correctly position the liquid medium between the two walls, the liquid medium then being located in the first area and outside the second area.

Growing community of inventors

Grenoble, France

Françoise Vinet