Geneohm Sciences Canada, Inc.

Genohm Sciences Canada, Inc.

Francois J Picard

Maurice Boissinot

Christian Menard

Michel G Bergeron

Marc Ouellette

Ann Huletsky

Paul H Roy

Martine Bastien

Jean Pierre Gayral

Jean-Pierre Gayral

Provided herein are compositions and methods for the detection of

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Compositions and methods for the detection of vancomycin-resistant pathogens using primers and/or probes to the vanA and vanB genes.

Methods and probes for detecting a vancomycin resistance gene

This invention relates to reagent comprising: any one of cells, viral particles, organelles, parasites, cells comprising organelles, cells comprising viral particles, cells comprising parasites, cells comprising bacterial cells and any combination thereof, the cells, viral particles, organelles or parasites comprising at least one nucleic acid sequence serving as an internal control (IC) target for nucleic acid testing (NAT) assay; wherein the reagent is suitable to be added to a test sample undergoing sample preparation to release, concentrate and/or purify nucleic acids and amplification and/or detection of nucleic acids so as to be used to verify: (i) the efficiency of sample preparation; and (ii) the efficiency of nucleic acid amplification and/or detection. The present invention also relates to a method to verify or validate the preparation and amplification and/or detection of a nucleic acid target sequence in a sample spiked with a reagent of the present invention.

Biological reagents and methods to verify the efficiency of sample preparation and nucleic acid amplification and/or detection

This invention describes a rapid (10 to 15 minutes), simple, flexible and efficient method of nucleic acids extraction for nucleic acid testing assays. This method has the following basic steps: i) mechanical cell lysis using solid particles in the presence of a chelating agent, followed by ii) controlling the presence and/or activity of NAT assays inhibitors. This method is applicable to various biological samples and universal for microorganisms, as one can use it to extract nucleic acids from test samples containing target viruses, bacteria, bacterial spores, fungi, parasites or other eukaryotic cells, including animal and human cells.

Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection

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Cap-Rouge, Canada

Francois J Picard