Sebia

Sebia, a Corp. of France

Laboratoires Sebia

Franck Bellon

Aline Bringard

The invention concerns a mask for distributing reagents on an analytical support, such as an electrophoresis gel, the mask having a body with parallel upper and lower surfaces, a plurality of lanes located on the lower surface of the mask have wedge-shaped projecting elements with a sloped surface between first and second ends thereof, an opening extends from the upper surface of the body of the mask to the lowest point of the sloped surface. The mask is positioned over an analytical support and loaded with the desired reagents which are held by capillary action between the mask and the analytical support. The mask can then be moved in a sweeping motion across the analytical support to deposit the reagents thereon. In another embodiment, the projecting elements are rectangular in shape and the mask is positioned at an incline with respect the analytical support.

Mask for depositing and distributing reagents on an analytical support

The invention concerns a process for separating alkaline phosphatase (ALP) isoenzymes from a biological sample by electrophoresis, characterized in that the electrophoresis is carried out on an electrophoresis support after depositing a solution of lectin onto the electrophoresis support in a predetermined localised zone, under conditions which permit interaction between said lectin and the ALP isoenzymes contained in the analysed biological sample, deposition of the lectin solution further being carried out under conditions which are suitable to allow separation of the ALP isoenzymes constituted by the osseous fraction and by the hepatic fraction.

Detection of alcaline isophosphatases by electrophoresis

The invention concerns a process for separating alkaline phosphatase (ALP) isoenzymes from a biological sample by electrophoresis, characterized in that the electrophoresis is carried out on an electrophoresis support after depositing a solution of lectin onto the electrophoresis support in a predetermined localized zone, under conditions which permit interaction between said lectin and the ALP isoenzymes contained in the analyzed biological sample, deposition of the lectin solution further being carried out under conditions which are suitable to allow separation of the ALP isoenzymes constituted by the osseous fraction and by the hepatic fraction.

The invention relates to a separation procedure for Lp(a) and the various lipoproteins contained in a biological sample by electrophoresis. The present invention is characterized in that the electrophoretic migration of the lipoproteins is carried out under conditions such that the electrophoresis gel and/or the above-mentioned biological sample contains compounds capable of modifying the electrophoretic mobility of Lp(a) differentially with respect to that of the other lipoproteins.

Separation procedure for Lp(a) by means of electrophoresis, gels for the

The object of the invention is an apparatus for the application of biological samples, in particular to an electrophoresis gel, comprising: --one or more planar elements, made of porous material, on which a biological sample is capable of being loaded, these planar elements resting in particular on the edge of a porous planar membrane, joint to the porous membrane and lying in an extention of the plane of the latter, and projecting with respect to the porous membrane, --these elements being designated hereafter as 'projecting elements'--, the part of the projecting elements not bound to the porous membrane having a free end, the above-mentioned projecting elements being such that they all have at least one point capable of being placed in contact with the planar surface of an electrophoretic slab support so that one or more biological samples can be loaded onto the said slab support, each biological sample being loaded beforehand on an element of the projecting elements, these different points being capable of being simultaneously placed in contact with the surface of the said slab support as a result of an alignment, when the above-mentioned porous membrane or the above-mentioned stiffening devices are arranged in an inclined or perpendicular plane with respect to the surface of the above-mentioned slab support.

Apparatus for the application of biological samples to an

A gel/mask set intended for the deposition, spreading and incubation of one or several liquid reagents on a gel layer (of total surface Sg) according to one or several well-defined zones of gel, here-after designated by 'incubation surface Si', this gel/mask set includes the gel layer itself and a rigid mask having a flat upper surface and a flat lower surface substantially parallel to the upper surface, at least one orifice intended to permit deposition and spreading of the liquid reagents by capillarity on the incubation surface Si of the gel layer, and at least one slit crossing the mask over the whole of its thickness and intended to permit withdrawal of any excess liquid reagent present on the incubation surface Si of the gel layer. Stops on the lower periphery of the mask rest on the gel layer and space the lower surface of the mask away from the gel layer by at least 0.1 mm. This invention is useful in staining for further study a gel layer previously subjected to a electrophoretic migration, particularly where the liquid reagent used for staining is expensive or must be conserved for other reasons.

Device permitting the spreading of one or several reagents on a gel

An apparatus for the application of one or more biological samples to an electrophoretic support is described. This apparatus features one or more planar projecting elements made of polyvinylidine difluoride or polysulfone having a perforated circular orifice within each projecting element for sample application. The planar projecting elements are attached to a common stiffening device for manual or automated application of the biological samples to a gel.

The invention relates to a rigid mask intended for the deposition, spreading and incubation of one or several liquids on a gel (of total surface Sg) according to one or several well-defined zones of the gel, hereafter designated by 'incubation surface Si', this mask comprising: an upper surface which is in particular flat and a lower surface which is in particular flat, in particular substantially parallel to each other, at least one orifice intended to permit deposition and spreading of the liquid on the incubation surface Si of the gel, and at least one slit crossing the mask over the whole of its thickness and intended to permit withdrawal of the excess of liquid present on the incubation surface Si of the gel,

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Longjumeau, France

Franck Bellon