Empresa Brasileira De Pesquisa Agropecuaria-embrapa

Basf Agrochemical Products B.v.

Fundação Universidade De Brasília

Francisco José Lima Aragão

Elibio Leopoldo Rech Filho

Ute Linemann

Dale R Carlson

Tim Malefyt

Bruce M Luzzi

Siyuan Tan

Tadashi Yotsumoto

Adolfo Ulbrich

Carlos Alberto Arrabal Arias

Luiz Louzano

Felipe Rodrigues Da Silva

Daniela Matias De Carvalho Bittencourt

Natalia Cristina Verza Ferreira

Pedro Ismael Da Silva Junior

Glaucia Barbosa Cabral

Giovanni Rodrigues Vianna

Luisa De Moraes Madeira

Natalia Lima De Sousa

Betúlia De Morais Souto

Aisy Botega Baldoni Tardin

Luiz Alberto Colnago

Paulo César Motta

Alan Carvalho Andrade

This invention relates to a method for obtaining castor-oil plants deprived of the ricin/RCA protein, through the insertion of gene constructs into plant cells, particularly castor-oil plant ones, with the consequent production of ricin/RCA-free castor-oil plant seeds. An aspect of the invention consists in providing castor-oil plants and parts thereof containing said gene construct. The method disclosed herein appeared to be efficient to the generation of castor-oil plants deprived of ricin protein or showing low expression levels of this protein, thus allowing the use of the seed thereof to the production of detoxified cakes, both for animal nutrition and for fertilizers, not to mention its possible production in countries that set restrictions to this substance, due to ricin toxicity. Therefore, this invention also provides a method and a kit to the identification of transformed plants containing the gene constructs disclosed herein.

Method for obtaining ricin/RCA-free castor-oil plant seeds, ricin/RCA-free castor-oil plants, method for identifying ricin/RCA-free castor-oil plants, polynucleotides, constructs and uses thereof

The Present invention relates to molecules isolated from the nucleic acid that encodes spider web proteins or fragments of these or other derivatives of these. The invention also refers to a chimerical gene and an expression vector containing molecules isolated from the nucleic acid that codes for proteins related to the webs ofandspiders. Another embodiment of the present invention are transformed cells containing a chimerical gene or an expression vector of the present invention. Yet another embodiment of the present invention relates to a method for obtaining genetically modified organisms containing inventive chimerical genes or expression vectors and a method for obtaining recombinant proteins from the silks ofandspiders. Finally, the invention describes products, such as biofilaments and compositions, using the recombinant proteins of the present invention. The discovery of new spider silk proteins, as well as their characterisation and expression in different heterologous systems shall be of great use in numerous areas, such as medicine and industry.

Proteins from the webs of nephilengys cruentata, a vicularia juruensis and parawixia bistriata spiders isolated from brazilian biodiversity

Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location comprises genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO:5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.

Soybean event 127 and methods related thereto

Compositions and methods related to transgenic AHAS-inhibiting herbicide resistant soybean plants are provided. Event 127 soybean plants having a mutated AHAS coding sequence which imparts tolerance to an AHAS-inhibiting herbicide are provided. The event 127 soybean plants having the event 127 nucleic acid molecule at the identified chromosomal location may comprise genomic/transgene junctions having at least the nucleic acid sequence of SEQ ID NO: 5 and/or 6. The characterization of the genomic insertion site of the event 127 provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event 127 soybean plants are provided.

Growing community of inventors

Brasília, Brazil

Francisco José Lima Aragão