Government of the United States of America, As Represented by the Secretary of Commerce

University System of Maryland

National Institute of Standards and Technology

The United States of America, As Represented by the Secretary of Commere, the National Institute of Standards and Technology

Francisco Javier Atencia-Fernandez

Laurie E Locascio

Gregory Alan Cooksey

Jack F Douglas

Susan Barnes

The present disclosure relates to devices and systems for separating motile pathogenic bacterial cells from samples. The disclosure also provides for methods of determining whether a sample is contaminated by pathogenic bacteria. The devices and systems disclosed herein are useful for screening water sources, environmental testing sites, food sources, and bodily fluids for the presence, absence, or quantity of bacterial cells in a sample representative of the screened sources.

Device and methods of using device for separation of bacteria from complex samples

A method and system for a folding microfluidic device comprises creating at least one folding line in a material, forming a plurality of layers of a microfluidic device in said material, and folding the material at the fold lines to create a self-aligned multilayered microfluidic device. The material can comprise a carrier material with an adhesive layer on the top and bottom surfaces.

Foldable microfluidic devices using double-sided tape

A magnetic connector assembly for microfluidic devices comprises a first magnetic connector with at least one orifice extending therethrough and a second magnetic connector. The first and second connectors are configured to magnetically attract each other. In one aspect, the first magnetic connector is configured to sealingly engage a surface of a microfluidic chip with the second magnetic connector disposed on an opposite side of the microfluidic chip. The first magnetic connector is configured to seal with the microfluidic chip about a channel opening in the microfluidic chip and provide flow communication between the channel opening and the orifice in the first magnetic connector. In at least one other aspect, the first magnetic connector and second magnetic connector each have at least one orifice and are configured to change a flow communication therebetween upon a rotation of the first or second magnetic connector with respect to the other magnetic connector.

Magnetic connectors for microfluidic applications

A method for microfabrication of a microfluidic device having sub-millimeter three dimensional relief structures is disclosed. In this method, homogeneous surfaces, which do not exhibit apparent pixel geometry, emerge from the interaction of the overlapping of diffracted light under opaque pixels and the nonlinear polymerization properties of the photoresist material. The method requires a single photolithographic step and allows for the fabrication of microstructures over large areas (centimeters) with topographic modulation of features smaller than 100 micrometers. The method generates topography that is useful in a broad range of microfluidic applications.

Fabrication method of topographically modulated microstructures using pattern homogenization with UV light

A microfluidic device is described, capable of generating multiple spatial chemical gradients simultaneously inside a microfluidic chamber. The chemical gradients are generated by diffusion, without convection, and can either be maintained constant over long time periods, or modified dynamically. A representative device is described with a circular chamber in which diffusion occurs, with three access ports for the delivery and removal of solutes. A gradient typically forms in minutes, and can be maintained constant indefinitely. Gradients overlapping with different spatial location, and a controlled rotation of a gradient formed by diffusion are demonstrated. The device can also be used to evaluate chemotactic responses of bacteria or other microorganisms in the absence of convective flow.

Growing community of inventors

Bethesda, MD, United States of America

Francisco Javier Atencia-Fernandez