Mead Johnson Nutrition Company

Mjn U.S. Holdings LLC

Francisco J Rosales

Gyan P Rai

Zeina Jouni

Robert J McMahon

Kristin Morris

Deborah A Schade

Eric Van Tol

Donald Carey Walker

Dirk Hondmann

Dattatreya Banavara

Rosaline Waworuntu

Nagendra Rangavajla

Juan M Gonzalez

William Michael Russell

Win-Chin Chiang

Yung-Hsiung Lee

Joaquin Franco

Hugh N Tucker

Deshanie Rai

Dattatreya Banavera

Zeina E Joani

Robert J Mcmahon

Zeina E Jouni

A nutritional composition for improving the immunological function of a subject, including a lipid or fat; a protein source; about 5 to about 100 mg/100 kcal of a source of long chain polyunsaturated fatty acids which comprises docosahexanoic acid; and about 0.1 to about 1 mg/100 kcal of a prebiotic composition, wherein the prebiotic composition comprises at least 20% of an oligosaccharide which has a fermentation rate which is demonstrably slower than that of galacto-oligosaccharide.

Nutritional composition to promote healthy development and growth

A nutritional composition having a lipid or fat; a protein source; a source of long chain polyunsaturated fatty acids which comprises docosahexanoic acid; and up to about 2.5% by weight of a source of supplemental calcium which, when dissociated, provides a cation having prebiotic functionality.

A novel method for determining the levels of TGF-&#x3b2;1 or TGF-&#x3b2;2 in a sample of milk, raw protein source, or nutritional composition is provided. The method involves, in some cases, reconstituting the sample; in some cases, centrifuging the sample; activating the sample using particular ratios of sample:acid:base; diluting the sample using particular ratios of sample:buffer agent; and determining the concentration of TGF-&#x3b2;1 in the sample using an ELISA assay.

Methods for determining the levels of TGF-&#x3b2; in a composition

A novel method for determining the bioactivity of TGF-&#x3b2; in a sample of milk, raw protein source, or nutritional composition is provided. The method includes particular reconstitution steps, centrifugation steps, incubation steps, and activation steps. The bioactivity of the TGF-&#x3b2; in the sample may be measured in a HT-2 cell bioassay or a cellomics bioassay.

Methods for determining the bioactivity of TGF-&#x3b2; in a composition

A nutritional formula which includes a lipid or fat; a protein source; about 5 to about 100 mg/100 kcal of a source of long chain polyunsaturated fatty acids which comprises docosahexanoic acid; about 0.1 to about 1 mg/100 kcal of a prebiotic composition, wherein the prebiotic composition comprises at least 20% of an oligosaccharide which comprises galacto-oligosaccharide; and about 0.015 to about 0.1 (pg/&#x3bc;g) ppm of TGF-&#x3b2;.

A nutritional composition including a lipid or fat; a protein source; at least about 5 mg/100 kcal of a source of long chain polyunsaturated fatty acids which comprises docosahexanoic acid; and at least about 0.2 mg/100 kcal of a prebiotic composition, wherein the prebiotic composition comprises a plurality of oligosaccharides such that the overall fermentation rate profile of the prebiotic composition provides an increased population of beneficial bacteria in the human gut over an extended period of time.

The present invention is directed an improved nutritional composition, methods of improving digestion, and methods of enhancing the bioavailability of TGF-&#x3b2;.

Nutritional composition with improved digestibility

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Singapore, Singapore

Francisco J Rosales