University of Missouri

Other

Smithkline Beecham Corporation

Francis J Schmidt

James T English

Zhiwei Fang

Gary Stacey

Mary E Brawner

James A Fornwald

Craig W Adams

Arnold L Smith

Roy O Morris

George P Smith

Sharon Bishop-Hurley

Bongrae Cho

Sharon L Bishop-Hurley

James E Schoelz

Farahnaz Rahmatpanah

Hugh B Nicholas, Jr

, the fungal pathogen that causes Asian soybean rust, has the potential to cause significant losses in soybean yield in many production regions of the U.S. To assist the development of new modes of soybean resistance to fungal infection, peptides were identified from combinatorial phage-display peptide libraries which inhibit germ tube growth from urediniospores of. Two peptides, Sp2 and Sp39, were identified that inhibit germ tube development when displayed as fusions with the coat protein of M13 phage or as fusions with maize cytokinin oxidase/dehydrogenase (ZmCKX1). These peptides may be used in vitro to help control fungal infection. These peptides may also be expressed as transgenes in a plant to help the resulting transgenic plant defend against the fungi.

Defense peptides against fungal infection and method of their use

A method for the identification of peptides having an affinity for the surface of fungi is provided as is a method for the identification of peptides capable of affecting the development of a fungus. Also provided are compositions comprising peptides identified using the method of the present invention. In addition, isolated polynucleotides, vectors, expression cassettes and transformed cells capable of expressing peptides identified by the method of the present invention are provided. Such polynucleotides, vectors, expression cassettes may be introduced into and confer upon plants the capability to resist fungal infection.

Method for inducing resistance to fungal infection in transgenic plants using plant defense peptides

The present invention relates generally to methods, kits, compositions, and combinations to identify anti-infective or anti-pathogenic agents. The present invention also relates to methods, kits, compositions, and combinations directed to identifying elements of RNA metabolism related to pathogen propagation, monitoring of these RNA metabolic events, and to agents capable of interrupting RNA metabolism in a pathogen-specific fashion.

Inhibitors of macromolecular activity

A method for the identification of peptides having an affinity for the surface of fungi is provided as is a method for the identification of peptides capable of affecting the development of a fungus. Also provided are compositions containing peptides identified using the method of the present invention. In addition, isolated polynucleotides, vectors, expression cassettes and transformed cells capable of expressing peptides identified by the method of the present invention are provided.

Phage display selection of anti fungal peptides

The instant invention relates to a method of inducing resistance to pathogens including fungi in transgenic plants by introducing into the plants a first sequence encoding a plant defense peptide and a second sequence encoding a peptide delivery scaffold.

Whole cell phage-display techniques were used to identify several peptides that bound preferentially to a non-typeable strain of. These peptides were able to inhibit growth of bothand. Thus, methods for treating bacterial infections, alone or in combination with traditional antibiotics, are envisioned.

[object Object]

RNA molecules are selected from a random sequence library for their ability to bind to a selecting nucleic acid structural element. Selection of RNA aptamers with extensive Watson-Crick complementarity to the nucleic acid ligand is precluded by inclusion of a blocking oligodeoxynucleotide in the binding phase of the selection protocol.

Method for obtaining RNA aptamers based on shape selection

A recombinant DNA molecule comprising the Streptomyces gal operon galK gene; galE gene; galT gene; P1 promoter; P2 promoter; P2 promoter expression unit; P1 promoter regulated region; or the entire Streptomyces gal operon.

Gal operon of streptomyces

A recombinant DNA molecule comprising the Streptomyces gal operon galK gene; galE gene; galT gene; P1 promoter, P2 promoter, P2 promoter expression unit; P1 promoter regulated region; or the entire Streptomyces gal operon.

Growing community of inventors

Columbia, MO, United States of America

Francis J Schmidt