Fmc Corporation

Francis H Kirkpatrick

Richard B Provonchee

Samuel Nochumson

Kenneth B Guiseley

Jonathan H Morgan

Foner P Curtis

Satyin Kaura

Henry A Daum, Iii

William Watt

T Chad Willis

Pegram A Johnson

Kenneth Guiseley

Henry A Daum

This invention provides electrophoresis gel container assemblies comprising at least a container element, a cover element, and means for releasably sealing the cover element to the container element; methods for fabricating and for using the container assemblies; and kits comprising the container assemblies with or without precast electrophoresis gels therein in combination with at least one electrophoresis auxiliary item.

Electrophoresis gel container assemblies

A three dimensional porous matrix, such as a reticulated open-cell foam, having within its matrix structure a mechanically-fractured hydrogel containing a network of fracture channels. The mechanically-fractured hydrogel may be a gel that has been partially dewatered by compression of the gel in situ. The porosity of the gel-in-matrix makes it useful for chromatographic applications and for immobilization of biologically-active components, where efficient, intimate contact of the hydrogel with a liquid medium is important.

Gel-in-matrix containing a fractured hydrogel

Polysaccharide resolving gels and gel systems for stacking

Purified agarose suitable for rapid electrophoresis, characterized by a sulfate content of less than 0.2 wt % but greater than zero, a pyruvate content of 0-0.1 wt %, and a nitrogen content of 0-0.02 wt %. Gels prepared from the agarose exhibit a gel strength at 1.0 wt % concentration of at least 1200 g/cm.sup.2, substantial absence of DNA binding in 0.07 M or less tris acetate buffer, and an electroendosmosis (EEO) at 1.0 wt % concentration of 0.05 or less. Agaroses are purified to provide the low EEO material by dissolving agarose or alkali-modified agar in an aqueous medium buffered at a pH of 6.0 to 8.0 and containing no more than 2.0 nM salt as chloride, and precipitating the agarose by contact with a lower alkanol.

High gel strength low electroendosmosis agarose

Growing community of inventors

Owls Head, ME, United States of America

Francis H Kirkpatrick