10x Genomics, Inc.

10x Gemomics, Inc.

Francesca Meschi

Xinying Zheng

Geoffrey McDermott

Zahra Kamila Belhocine

Jason Bell

Michael Schnall-Levin

Katherine Pfeiffer

Josephine Lee

Andrew D Price

Tobias Daniel Wheeler

Yifeng Yin

Luz Montesclaros

Jerald Sapida

Kamila Belhocine

Jessica Michele Terry

David Sukovich

Solongo B Ziraldo

Joseph Francis Shuga

Corey Nemec

Geoffrey Mcdermott

Samuel Marrs

The present disclosure provides methods and systems for sample preparation and/or analysis. Samples may be cells, or may be derived from one or more cells. Sample preparation may comprise conducting one or more reactions on a target. Such reactions may be conducted in one or more partitions. One or more reactions may be performed in one or more successive operations.

Methods and systems for sample preparation and analysis

Methods and compositions to minimize barcode exchange during the preparation of barcoded next-generation sequencing libraries prepared from a single cell. The methods utilize oligonucleotides containing a 3'-terminated blocking group or sequences that prevent amplification or extension.

Methods and systems to minimize barcode exchange

The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

Methods and systems for analysis of chromatin

Methods and systems for sample preparation techniques that allow amplification (e.g., whole genome amplification) and sequencing of chromatin accessible regions of single cells are provided. The methods and systems generally operate by forming or providing partitions (e.g., droplets) including a single biological particle and a single bead comprising a barcoded oligonucleotide. The preparation of barcoded next-generation sequencing libraries prepared from a single cell is facilitated by the transposon-mediated transposition and fragmentation of a target nucleic acid sequence. The methods and systems may be configured to allow the implementation of single-operation or multi-operation chemical and/or biochemical processing within the partitions.

Single cell analysis of transposase accessible chromatin

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Menlo Park, CA, United States of America

Francesca Meschi