Qiagen Gmbh

Qiagen Manchester Limited

Francesca Di Pasquale

Holger Engel

Nicola Jo Thelwell

Alexander Azzawi

Andy Wende

Sabine Werner

Sascha Strauss

Daniel Müller

Sascha Strauß

The present invention relates to a method for quantifying and/or detecting one or more nucleic acids of a genome in a sample, wherein in an amplification reaction, (i) a first nucleic acid is amplified, the locus that is amplified is a multicopy locus (MCL) within the genome, wherein the locus shares at least 80% sequence identity to a sequence according to SEQ ID NO. 1 over a stretch of 80 base pairs, and wherein the multicopy locus has copies on at least two different chromosomes, (ii) a second nucleic acid that has been added as an internal control (IC) is also amplified, and (iii) the amount of amplification product from the amplification of the first nucleic acid is determined.

Method for quantifying human DNA using an internal control

The invention provides for a method for quantifying one or more nucleic acids of a genome in a sample comprising the steps of, (a) amplifying a first nucleic acid to be quantified, (b) determining the amount of said first nucleic acid by comparison of the amount of amplification product from said first nucleic acid with at least one amplification product from a second template nucleic acid, (c) wherein said second template nucleic acid was generated using whole genome amplification and wherein the starting concentration of the second template nucleic acid is known.

Method for quantifying human DNA

The present application discloses a labeled nucleotide comprising a label attached via a linker, wherein said labeled nucleotide has the formula wherein Ris a residue with a negative net charge, preferably selected from the group consisting of a phosphate group, and a sulphate group; wherein R, Rand Rare independently selected from the group consisting of H, OH, and O; wherein 'n' is an integer between 0 and 16; wherein 'a' is an integer between 1 and 10; wherein SP is absent or a spacer; wherein X is said label; and wherein Y is a nucleotide or nucleoside. Furthermore, oligonucleotides comprising a labeled nucleotide according to the present invention and the use as a primer in amplification based methods is disclosed herein. 

Oligonucleotides comprising a label associated through a linker

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Hilden, Germany

Francesca Di Pasquale