California Institute of Technology

Gevo, Inc.

The Scripps Research Institute

The United States of America As Represented by the Department of Energy

Dow Silicones Corporation

U.S. Department of Energy

Maxygen, Inc.

Frances H Arnold

Peter Meinhold

Matthew W Peters

Rudi Fasan

Mike M Y Chen

Zhixin Shao

Ulrich Schwaneberg

Edgardo Farinas

Anton Glieder

Jared C Lewis

Lorraine Joan Giver

Zhan Wang

Eric M Brustad

Pedro S Coelho

Pete Heinzelman

Kai Chen

Alexander Volkov

Sabine Bastian

Huimin H Zhao

Ioanna P Petrounia

Stephen R Quake

Joseph A Affholter

Stephen L Mayo

Darryl Y Sasaki

Anne Y Fu

Charles F Spence

Chi-Huey Wong

Karlheinz Drauz

Dimitris Katsoulis

Viviana Gradinaru

Aristos Aristidou

Zhibin Guan

Andreas Bommarius

Kevin Yang

Catherine Asleson Dundon

Jun Urano

Andrew Hawkins

William H Greenberg

John Roberts

Oliver May

Sabine Brinkmann-Chen

Doug Lies

Thomas Buelter

Reid M Renny Feldman

Kent Evans

Julie Kelly

Stephanie Porter-Scheinman

Patrick C Cirino

S B Jennifer Kan

Ruth Berry

Melissa Dey

Justas Jancauskas

Russell D Lewis

Lianhong Sun

Christopher C Farwell

John A McIntosh

Daniel J Koch

Deborah Shnek

Sek Bik Jennifer Kan

Jeffrey C Moore

Toni M Lee

Zhanglin Lin

Patrick J Almhjell

Pradeep Dhal

Keqin Chen

Ruijie Zhang

Devin L Trudeau

Guohua Chen

Sean Plunkett

Gerald E Wuenschell

Daniel Pack

Vidyasankar Sundaresan

Claire N Bedbrook

Susanne Baehr

Lena Wohlschlager

Indira Wu

Devin Trudeau

Yuuichi Mitsuda

Michael M Chen

Clay Bennett

Anton Blieder

Christopher R Otey

Lian-Hong Sun

Hyun Joo

Hans H Renata

Chao-Tsen Chen

Eva Eckl

Reid M Feldman

Matthew Peters

Provided herein is an engineered tryptophan synthase β-subunit (TrpB) that catalyzes the synthesis of tyrosine, tyrosine analogs, or salts thereof. Also provided herein are methods for preparing tyrosine, tyrosine analogs, or a salt thereof using the engineered TrpB described herein.

Engineered enzymes and method for the synthesis of diverse tyrosine analogs

This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.

Alkane oxidation by modified hydroxylases

This disclosure provides a method of preparing a silanol-functional organosilicon compound with a cytochrome P450 variant that facilitates the oxidization of a silyl hydride group to a silanol group in the presence of oxygen. The method includes combining the cytochrome P450 variant and an organosilicon compound having at least one silicon-bonded hydrogen atom to give a reaction mixture and exposing the reaction mixture to oxygen to oxidize the organosilicon compound, thereby preparing the silanol-functional organosilicon compound. Cytochrome P450 variants suitable for use in the method are also disclosed, along with methods for engineering and optimizing the same. Nucleic acids encoding the cytochrome P450 variants and compositions, expression vectors, and host cells including the same are also disclosed.

Method of preparing silanols with selective cytochrome P450 variants and related compounds and compositions

Disclosed herein are engineered light-sensitive proteins, for example channelrhodopsins and variants thereof. Also disclosed are compositions for expressing the light-sensitive proteins in cells, tissues, organs and subjects, and methods for using the light-sensitive proteins to, for example, enable minimally-invasive neuronal circuit interrogation in living organism, and treat neuronal and ocular disorders.

Engineered light-sensitive proteins

The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.

Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes

Methods for catalyzing C&#x2014;H insertion reactions using heme enzymes are described. The present disclosure provides a method for producing a C&#x2014;H insertion product comprising providing an substrate having an sp-hybridized C&#x2014;H bond, a carbene precursor such as a diazo reagent, and a heme enzyme, and admixing the components in a reaction for a time sufficient to produce the C&#x2014;H insertion product. Heme enzyme variants useful for carrying out in vivo and in vitro C&#x2014;H insertion reactions, as well as expression vectors and host cells expressing the heme enzymes, are also described.

Method for enantioselective carbene C&#x2014;H insertion using an iron-containing protein catalyst

The present invention provides compositions and methods for catalyzing the formation of carbon-silicon bonds using heme proteins. In certain aspects, the present invention provides heme proteins, including variants and fragments thereof, that are capable of carrying out in vitro and in vivo carbene insertion reactions for the formation of carbon-silicon bonds. In other aspects, the present invention provides methods for producing an organosilicon product, the method comprising providing a silicon-containing reagent, a carbene precursor, and a heme protein; and combining the components under conditions sufficient to produce an organosilicon product. Host cells expressing the heme proteins are also provided by the present invention.

Method for producing an organosilicon product

The disclosure generally relates to the fields of synthetic organic chemistry. In particular, the present disclosure relates to methods and systems for the imidation of sulfides.

Methods and systems for sulfimidation or sulfoximidation of organic molecules

In vivo and in vitro olefin cyclopropanation catalyzed by heme enzymes

Cytochrome C protein variants for catalyzing carbon-silicon bond formation

The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides.

Stable, functional chimeric cellobiohydrolase class I enzymes

Growing community of inventors

La Canada, CA, United States of America

Frances H Arnold