Biomerieux, Inc.

Fran&ccedil;ois Mallet

Bernard F Mandrand

Guy Oriol

Alain Theretz

William McAllister

Val&eacute;rie Cheynet-Sauvion

Nad&eacute;ge Arnaud-Barbe

Christine Hebrard

Eric Perouzel

Disclosed is a process for transcribing RNA using a nucleotide reagent as the promoter. Such a reagent enables any type of RNA to be transcribed without sequence specification and without protein cofactors, by means of an RNA polymerase that is known to be DNA-dependent such as the RNA polymerase of the phage T7, or by means of new, mutated RNA polymerase with the ability to synthesize a transcription product of a polynucleotide matrix with a higher yield when the matrix is RNA than when said matrix is DNA. This type of RNA polymerase can be obtained by effecting mutations on a coding gene for a wild-type RNA polymerase, and then by selecting the mutated RNA polymerase with said ability. The invention can be applied notably to the detection, synthesis or quantification of RNA.

Method of amplifying an RNA target sequence using an RNA polymerase functioning preferably on RNA template

The invention concerns a method for fixing a biological molecule, associated with a specific binding site, on the surface of a support made of silica or metal oxide. The invention also concerns a surface functionalized by the method and the use of such a surface. The method is characterized in that it consists in: functionalizing the surface by cleaning, using at least a solvent or an oxygen plasma or any other process for forming alcohol groups on the support surface, to make it hydrophilic; contacting the biological molecule on said functionalized surface; and functionalizing the support by fixing the biological molecule specific binding site on at least one of the support alcohol groups. The invention is particularly useful in the field of biomedical diagnosis.

Attaching a biological molecule to a support surface

Growing community of inventors

Villeurbanne, France

François Mallet

Growing community of inventors

Villeurbanne, France

Fran&ccedil;ois Mallet

François Mallet