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Centre National De La Recherche Scientifique

Institut Pasteur and Centre National De La Recherche Scientifique

Institut Pasteur Centre National De La Recherche Scientifique

Fran&ccedil;ois Heslot

David Bensimon

Aaron Bensimon

Ulrich Bockelmann

Baptiste Essevaz-Roulet

Molecular combing process for detecting macromolecules

The invention concerns a method for characterizing nucleic acid duplexes comprising two sequences of nucleic acid at least partially paired, characterized in that it consists in recording at least a signature of said duplexes, which is linked to the force variation required for separating, receptively re-pairing, said two sequences, and in comparing the resulting signature with references.

Method for characterizing nucleic acid duplex

A process for aligning a macromolecule on the surface S of a support comprises forming a triple line S/A/B (meniscus) resulting from contact between a solvent A and the surface S and a medium B is caused to move on the surface S. The macromolecule has a part, especially an end, anchored on the surface S. The other part, especially the other end, is in solution in the solvent A. A process for detecting, measuring the intramolecular distance of, separating and/or assaying a macromolecule in a sample using the process of alignment is provided.

Process for aligning macromolecules by passage of a meniscus and applications

The invention relates to a process of attaching an end of a nucleic acid on a surface by providing a solution having a pH that allows for specific attachment of an end of a nucleic acid to a surface. The surface, the solution, and the nucleic acid are contacted to attach the end of the nucleic acid to the surface.

Processing for attaching an end of a nucleic acid to a surface by utilizing pH

A process for aligning a macromolecule (macromolecules) on the surface S of a support is characterized in that the triple line S/A/B (meniscus) resulting from the contact between a solvent A and the surface S and a medium B is caused to move on the said surface S, the said macromolecules having a part, especially an end, anchored on the surface S, the other part, especially the other end, being in solution in the solvent A. The subject of the present invention is also a process for detecting, measuring the intramolecular distance of, separating and/or assaying a macromolecule in a sample in which a process of alignment according to the invention is used.

Growing community of inventors

Viroflay, France

François Heslot

Growing community of inventors

Viroflay, France

Fran&ccedil;ois Heslot

François Heslot