Icon Genetics Ag

Fong-Chin Huang

Christian Eibl

Stefan Mühlbauer

Hans-Ulrich Koop

Sebastian Klaus

Stefan Herz

Timothy J Golds

A process of generating transgenic plants or plant cells transformed on their plastome and being devoid of a selection marker, comprising the following steps: (a) transforming plastids of a plant or plant cell with a DNA comprising: (i) a nucleotide sequence conferring replication of said DNA in a plant cell, (ii) at least one sequence of interest, (iii) sequences flanking said at least one sequence of interest necessary for stable integration of said at least one sequence of interest into the plastid genome, and (iv) a selection marker outside of said sequences flanking said sequence(s) of interest; (b) allowing integration of said at least one sequence of interest into the plastome in the presence of selective pressure; (c) allowing loss of said selection marker sequence by releasing selective pressure; and (d) recovering cells and/or plants being genetically transformed on their plastomes and being devoid of said selection marker.

Process of generating transplastomic plants or plant cells devoid of a selection marker

A process for producing multicellular plants, plant organs or plant tissues transformed on their plastome by the following steps is provided: (a) altering or disrupting the function of a gene in a plastid genome for producing a selectable or recognizable phenotype; (b) separating or selecting plants or cells having plastids expressing said phenotype; (c) transforming said plastid genome of said separated or selected plant, plant organ or plant tissue with at least one transformation vector having a restoring sequence capable of restoring said function; and (d) separating or selecting said transformed plant, plant organ or plant tissue having plastids expressing said restored function.

Processes and vectors for plastid transformation of higher plants

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Taipei, Taiwan

Fong-Chin Huang