Illumina Corporation

Leland Stanford Junior University

Foad Mashayekhi

Tarun Kumar Khurana

Kevin L Gunderson

Frank J Steemers

Mostafa Ronaghi

Yir-Shyuan Wu

Jeffrey S Fisher

Arash Jamshidi

Xi-Jun Chen

Filiz Gorpe-Yasar

Charles Lin

Hamed Amini

Hei Ka Patrick Tam

Pallavi Daggumati

Falko Noé

Falko Noe

Disclosed herein is a novel method of producing monodisperse aqueous droplets, as well as a novel microfluidic droplet generator. In some examples, the method comprises flowing an aqueous solution through a microchannel and into a sample reservoir of the microfluidic droplet generator, wherein monodisperse droplets of the aqueous solution form by step-emulsification at a step change in height at an intersection of a reservoir end of the microchannel and a sidewall of the sample reservoir. In some examples, the aqueous solution is a hydrogel precursor solution and monodisperse droplets of the hydrogel precursor solution form by step-emulsification at the step change in height at the intersection of the reservoir end of the microchannel and the sidewall of the sample reservoir. In some examples, the monodisperse droplets of the hydrogel precursor solution are incubated under conditions suitable for gelation to form hydrogel beads.

Microfluidic droplet generators

Described are microfluidic devices and methods for providing a predetermined number of microspheres or beads, together with a cell, within a fluid droplet being processed. The system may provide each droplet with a single bead and a single cell, and the bead may contain DNA or other reagents for later identifying the specific cell associated with that bead.

Inertial droplet generation and particle encapsulation

Described herein are methods and compositions relating to amplifying nucleic acid. In certain embodiments, the invention provides methods for labeling and amplifying a nucleic acid molecule.

Methods and compositions for clonal amplification of nucleic acid

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San Francisco, CA, United States of America

Foad Mashayekhi