University of California

The United States of America As Represented by the Department of Health

National Institutes of Health, a Component of the US Dept. of Health & Human Services

Other

The United States of America As Represented by the Secretary of

The United States of America

Hoffmann-la Roche Inc.

Dana-farber-cancer Institute Inc.

Centocor, Inc.

The Government of the United States of America, As Represented by the

Flossie Wong-Staal

Robert C Gallo

Nancy T Chang

Gunter Kraus

Mang Yu

George M Shaw

Carl W Saxinger

Osamu Yamada

Eric M Poeschla

Joshua O Ojwang

Syed Zaki Salahuddin

Robert M Crowl

Mark Leavitt

Anthony Ho

E Prem Reddy

Dharam V Ablashi

Steven F Josephs

Takis S Papas

Markley C Leavitt

James A Lautenberger

Pierre Corbeau

Kenneth Samuel

Randy L Talbott

Michael Mamounas

John Ghrayeb

Joseph G Sodroski

William A Haseltine

Beatrice H Hahn

James R Rusche

Mikulas Popovic

Edgar P Heimer

Genoveffa Franchini

Craig A Rosen

Jay F Rappaport

Steven Francis Josephs

Premkumar E Reddy

Pranab K Chanda

Amanda G Fisher

Suresh K Arya

Syed Z Salahuddin

David J Looney

The determination of the nucleotide sequence of HIV-1 DNA; identification, isolation and expression of HIV-1 DNA sequences which encode immunoreactive polypeptides by recombinant DNA methods and production of viral RNA are disclosed. Such polypeptides can be employed in immunoassays to detect HIV-1.

Cloning and expression of HIV-1 DNA

Disclosed is the molecular cloning of HTLV-III, the adult leukemia and acquired immune deficiency syndrome (AIDS) virus. Clone BH10 contains a 9.0 Kb viral insert constituting the entire HTLV-III genome. Clones BH8 and BH5 contain viral inserts of 5.5 Kb and 3.5 Kb, respectively. These clones are suitable for the development of diagnostic and therapeutic measures for AIDS, as well as use as probes for the detection of AIDS. By scientific convention, HTLV-III, referred to herein also as HIV, has been renamed as HIV-1.

Molecular cloning of HIV-1 from immortalized cell lines

The determination of the nucleotide sequence of HTLV-III DNA; identification, isolation and expression of HTLV-III sequences which encode immunoreactive polypeptides by recombinant DNA methods and production of viral RNA are disclosed. Such polypeptides can be employed in immunoassays to detect HTLV-III.

Cloning and expression of HTLV-III DNA

A new human B lymphotropic virus, also designated human herpesvirus-6, has been isolated. DNA, molecular clones, antigenic viral proteins and antibodies having specificity to the new virus have been prepared. Various utilities of the new virus and products derived therefrom have been described.

Antibodies against human herpes virus-6(HHV-6) and method of use

The determination of the nucleotide sequence of HTLV-III DNA; identification, isolation and expression of HTLV-III sequences which encode immunoreactive polypeptides by recombinant,DNA methods and production of viral RNA are disclosed. Such polypeptides can be employed in immunoassays to detect HTLV-III.

Detection of HIV-1 DNA

The invention provides non-primate lentiviral vectors, packaging cells and packaging plasmids based, for example, on feline and ungulate retroviruses. In particular, the packaging plasmids are designed for expression in human cells (which are also used as packaging cells). The vectors of the invention transduce human cells, including difficult to target non-dividing cells of the hematopoietic and nervous system, in vitro and in vivo. The vectors are suitable for general gene transfer to these cells and for gene therapy to treat conditions mediated by these non-dividing cells including cancer and HIV infection.

Lentiviral nucleic acids and uses thereof

By transducing cells with an HIV-1-MN molecular clone deleted in the major packaging sequence, a stable HIV-1 packaging cell line, &psgr;422 was produced. &psgr;422 cells form syncytia with CD4 positive cells, correctly express HIV-1 structural proteins, and produce large amount of mature particles with normal RT activity. These particles are not infectious. When stably transfected with an HIV-based retroviral vector, the &psgr;422 cell line produces hybrid virions capable of transducing CD4 positive cells with high efficiency (e.g., 10,cells/ml). The availability of this stable, noninfectious HIV-1 packaging cell line capable of generating high titer HIV vectors enables the use of HIV-1 based nucleic acids delivery systems, for example, in gene therapy. An HIV-2 based vector is packaged by the packaging cell lines, demonstrating that HIV-2 cell transformation vectors are packaged by the packaging cell line. HIV based vectors packaged by the high efficiency cell lines are shown to have anti-HIV activity per se.

Design of novel highly efficient HIV based packaging systems for gene therapy

Novel HIV-2 proviruses, molecular clones, nucleic acids, polypeptides, viruses and viral components are described. The use of these compositions as components of diagnostic assays, as immunological reagents, as vaccines, as components of packaging cells, cell transduction vectors, and as gene therapy vectors is also described.

Polypeptides encoded by novel HIV-2 proviruses

Cell transformation vector comprising an HIV-2 packaging site nucleic acid and an HIV-1 GAG protein

This invention provides an infectious retrovirus having inserted between the 5' and 3' long terminal repeat sequences of the retrovirus a nucleic acid encoding an anti-HIV-type specific agent or a foreign nucleic acid under the control of a pol III promoter. Host cells containing the retroviral vectors of this invention also are provided. Further provided are methods of interfering with or preventing HIV viral replication in a cell infected with HIV or likely to be infected with HIV.

Retroviral vectors comprising an anti-hiv or other nucleic acid

An envelope protein of the etiologic agent of acquired immune deficiency syndrome (AIDS) and a method for its preparation are disclosed. Proviral DNA is transferred into a host cell after engineering into an expression vector which produces the envelope protein. A method of testing human blood for the presence of antibodies to the AIDS virus using the AIDS envelope protein is disclosed.

Acquired immune deficiency syndrome (AIDS) viral envelope protein and

Antibodies against human herpesvirus-6(HHV-6) and method of use

Human herpesvirus-6 (HHV-6) isolation and products

Growing community of inventors

San Diego, CA, United States of America

Flossie Wong-Staal