Genentech, Inc.

Florian M Wurm

Martin Jordan

Phillip W Berman

Brian M Fendly

Timothy J Gregory

The invention provides antibodies specific for HIV env, including monoclonal antibodies and related hybridomas. The antibodies block CD4/g120 binding and reduce reverse transcriptase activity in vitro.

HIV env antibodies

Method for enhancing gene expression

Methods for producing and isolating unclipped HIV env proteins are provided. According to the methods, an antibody directed to an HIV epitope spanning the env clip site is used to selectively separate unclipped HIV env protein from clipped env protein.

Method for isolation of unclipped HIV envelope protein

A method is provided for calcium phosphate transfection of a eukaryotic host cell wherein particles comprising calcium phosphate and a desired nucleic acid are grown to an optimal size and then contacted with the host cell under conditions providing a substantially slower particle growth rate, thereby increasing the host cell's exposure to optimally-sized particles.

Methods for calcium phosphate transfection

A method is provided for calcium phosphate transfection of a eukaryotic host cell in suspension wherein particles comprising calcium phosphate and a desired nucleic acid are grown to an optimal size and then contacted with the host cell in suspension under conditions that substantially reduce the particle growth rate and allow the host cell to take up the particles to form a transfected cell in suspension.

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Redwood City, CA, United States of America

Florian M Wurm