Hoffmann-La Roche Inc.

Florian Lipsmeier

Hubert Kettenberger

Stefan Klostermann

Apollon Papadimitriou

Jasmin Sydow-Andersen

Herein is reported a method for selecting or deselecting an antibody comprising a) determining for each Asp and Asn residue in an antibody Fv region the conformational flexibility of the C-atom using a homology model ensemble, b) determining for each Asp and Asn residue in an antibody Fv region the size of the amino acid residue C-terminal to the Asp or Asn residue, and c) selecting an antibody in which the C-atom is conformationally inflexible and/or the Asp or Asn has a big C-terminal amino acid residue, or deselecting an antibody in which the C-atom has a moderate to high conformational flexibility and/or the Asp or Asn has a small C-terminal amino acid residue.

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Penzberg, Germany

Florian Lipsmeier