Other

Kern, Florian

Florian Kern

Hans-Dieter Volk

Petra Reinke

Elham Khatamzas

Nicole Faulhaber

Ingolf-Pascal Surel

Peter Walden

Alexander Scheffold

Rainer Blasczyk

The invention relates to a method for identifying T-cell stimulating protein fragments using the following steps: a) detecting the amino acid sequence of an antigen; b) subdividing the found amino acid sequence of the antigen into protein fragments; c) synthesizing at least one protein fragment; d) incubating a suspension containing t-cells with the protein fragments; e) identifying an induced T-cell cytokine or activation marker by flow-through cytometry, and; f) assigning the T-cells, with which T-cell cytokines and/or activation markers were identified, to the protein fragments which were incubated with the T-cells. The corresponding protein fragments/peptides are synthetically produced with the assistance of the detected positive sequence, and said corresponding protein fragments/peptides can be utilized to produce a medicament for immunostimulation.

Method for identifying t-cell stimulating protein fragments

The present invention relates to peptides or pretide derivatives of the 65kD lower matrix phosphoprotein of human cytomegalovirus. The peptides disclosed are useful for determining whether subjects have een exposed to human cytomegalovirus. Methods for using these peptides or derivatives thereof to determine the peptide-induced production of interferon-&#x3b3; and/or TNF-&#x3b1; in CD8T cells isolated from subjects are also disclosed.

Peptides for vaccination against human CMV

The invention relates to a method for the antigen-specific stimulation of T-lymphocytes with a synthetic peptide library, by preparing a plurality of peptides, each of said peptides comprising a fragment less than the whole of the total amino acid sequence of the antigen, each of said peptides being a minimum of 9 amino acid residues (AAs) in length, and the amino acid sequence of each peptide overlapping with the amino acid sequence of at least one other of said peptides; combining a plurality of the peptides from a) into a peptide library, said peptide library comprises a plurality of the peptides selected such that their collective overlapping amino acid sequences span the total amino acid sequence of the antigen; and incubating a suspension comprising CD8+ T lymphocytes, CD4+ T lymphocytes or a mixture of CD8+ and CD4+ with said peptide library in a single culture run.

Method for antigen-specific stimulation of T lymphocytes with synthetic peptide libraries

The present invention relates to peptides or peptide derivatives of the 65 kD lower matrix phoshpoprotein of human cytomegalovirus. The peptides disclosed are useful for determining whether subjects have been exposed to human cytomegalovirus. Methods for using these peptides or derivatives thereof to determine the peptide-induced production of interferon-&#x3b3; and/or TNF-&#x3b1; in CD8T cells isolated from subjects are also disclosed.

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Berlin, Germany

Florian Kern