Roche Molecular Systems, Inc.

Cea/institut De Genomique—centre National De Genotypage

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Centre National De Genotypage

Florence Mauger

David H Gelfand

Keith Bauer

Keith A Bauer

Ivo Glynne Gut

The present application provides polynucleotides comprising 5'-tails with sequence segments useful for the detection of target nucleic acid sequences, and methods for their use in detecting target nucleic acids. The polynucleotides are used to amplify a subsequence of a target nucleic acid in the presence of one or more ribonucleotides. The ribonucleotides are incorporated into amplification products at regular intervals complementary to the 5&#x2032;-tail sequence segments. Cleavage of amplification products at the bond immediately 3&#x2032; to incorporated ribonucleotides produces detectably distinct fragments indicative of the presence or absence of a target nucleic acid.

Ribonucleotide tag nucleic acid detection

Growing community of inventors

Evry, France

Florence Mauger