Cetus Corporation

Other

Flint Smith

Robert F Halenbeck

Glenn J Dorin

Michael G Kunitani

Sidney N Wolfe

Maninder Singh

John T Davis

Robert Weissburg

Amy Lim

Compositions are described that are suitable for formulating cytokines, preferably tumor necrosis factor, that maintain their biological activities over a wide range of temperatures by, among other aspects, decreasing them sensitivity to agitation, and preventing oligomer and particulate matter formation.

Tumor necrosis factor formulations

A process for recovering highly pure, recombinant IL-2 from transformed microorganisms in which the cells are disrupted; impure recombinant IL-2 is isolated in the form of refractile bodies from the disruptate; the impure IL-2 is dissolved and denatured with at least 6M guanidine hydrochloride containing a reducing agent; the reduced IL-2 is precipitated and resolubilized; the reduced solubilized IL-2 therein is oxidized by a controlled oxidation; the oxidized IL-2 is refolded by reducing the concentration of guanidine hydrochloride in the solution; and the oxidized, refolded IL-2 is further purified by ion exchange chromatography or hydrophobic interaction chromatography and ion exchange chromatography.

Process for recovering purified, oxidized, renatured recombinant

A process for recovering substantially pure rIL-2 from transformed microorganisms in which the cells are disrupted, impure insoluble rIL-2 is separated from the bulk of the cellular components, the separated impure rIL-2 is solubilized and partially purified in a reduced form, the solubilized rIL-2 is oxidized, the oxidized rIL-2 is purified to clinically acceptable levels, and the oxidized, purified IL-2 is denatured by placing it into a solution of a chaotropic agent, solids are removed from the solution and rIL-2 is renatured from the solution.

Process for recovering microbially produced interleukin-2

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Oakland, CA, United States of America

Flint Smith