Illumina Corporation

Fluidigm Corporation

Fiona Kaper

Andrew Paul May

Peilin Chen

Megan Anderson

Jian-Bing Fan

Brian Fowler

Gordon M Cann

Jun Wang

Ronald Lebofsky

Robert C Jones

Sarah L Kinnings

Suzanne Rohrback

Sarah E Shultzaberger

Carlo Randise-Hinchliff

Kevin L Gunderson

Frank J Steemers

Dmitry K Pokholok

Thomas Royce

Craig April

Tarun Kumar Khurana

Jerome J Jendrisak

Yir-Shyuan Wu

Jeffrey S Fisher

Alex Aravanis

Sasan Amini

Arash Jamshidi

Igor Yu Goryshin

Nicholas Caruccio

Mark A Maffitt

Tong Liu

Natasha Pignatelli

Clifford Lee Wang

Hongxia Xu

Neeraj Salathia

Dan Cao

Shi Min Xiao

Burak Okumus

Victor J Quijano

Casey Turk

Reza Kahlor

Reza Kalhor

A method of preparing a library of tagged nucleic acid fragments including contacting a population of cells directly with a lysis reagent having one or more protease to generate a cell lysate; inactivating the protease to generate an inactivated cell lysate, and applying a transposase and a transposon end composition containing a transferred strand to the inactivated cell lysate under conditions wherein the target nucleic acid and the transposon end composition undergo a transposition reaction.

Library preparation of tagged nucleic acid using tube add-on protocol

The present invention includes methods and materials for use in the detection preeclampsia and/or determining an increased risk for preeclampsia in a pregnant female, the method including identifying in a biosample obtained from the pregnant women a plurality of circulating RNA (C-RNA) molecules.

Circulating RNA signatures specific to preeclampsia

The present disclosure relates to systems and methods for the amplification of nucleic acids, including, but not limited to, the amplification of nucleic acid libraries and whole genome amplification.

Compositions and methods for the amplification of nucleic acids

An example of a biotin-streptavidin cleavage composition includes a formamide reagent and a salt buffer. The formamide reagent is present in the biotin-streptavidin cleavage composition in an amount ranging from about 10% to about 50%, based on a total volume of the biotin-streptavidin cleavage composition. The salt buffer makes up the balance of the biotin-streptavidin cleavage composition. In some examples, the biotin-streptavidin cleavage composition is used to cleave library fragments from a solid support. In other examples, other mechanisms are used to cleave library fragments from a solid support.

Biotin-streptavidin cleavage composition and library fragment cleavage

Described herein are methods useful for incorporating one or more adaptors and/or nucleotide tag(s) and/or barcode nucleotide sequence(s) one, or typically more, target nucleotide sequences. In particular embodiments, nucleic acid fragments having adaptors, e.g., suitable for use in high-throughput DNA sequencing are generated. In other embodiments, information about a reaction mixture is encoded into a reaction product. Also described herein are methods and kits useful for amplifying one or more target nucleic acids in preparation for applications such as bidirectional nucleic acid sequencing. In particular embodiments, methods of the invention entail additionally carrying out bidirectional DNA sequencing. Also described herein are methods for encoding and detecting and/or quantifying alleles by primer extension.

Nucleic acid encoding reactions

In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

Multi-primer amplification method for barcoding of target nucleic acids

Library preparation of tagged nucleic acid

Presented herein are methods and compositions for multiplexed single cell gene expression analysis. Some methods and compositions include the use of droplets and/or beads bearing unique barcodes such as unique molecular barcodes (UMI).

Nucleic acid sequence analysis from single cells

Presented herein are methods and compositions for determining haplotypes in a sample. The methods are useful for obtaining sequence information regarding, for example, HLA type and haplotype. Also presented herein are methods of determining haplotypes in a sample based on a plurality sequence reads.

HLA typing using selective amplification and sequencing

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San Diego, CA, United States of America

Fiona Kaper