Life Technologies Corporation

Ferrier Le

Mark R Andersen

Michael C Pallas

Jian Gong

Kornelija Zgonc

Marcin Sikora

Gordon A Janaway

Casey R McFarland

Haopeng Wang

Gothami Padmabandu

Tom Cheng Xu

David Dupont

Christopher Trinh

Yating Shi

Claire Marjoribanks

Jordan Lang

Kalpith Ramamoorthi

Junko Stevens

Nicole Fantin

Joyce Wilde

Casey Mcfarland

Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

System and method for determining copies-per-unit-volume using PCR and flow control of droplets

A composition comprising a thermostable DNA polymerase; and a PCR inhibitor blocking agent, wherein the PCR inhibitor blocking agent is present in an amount effective to enhance tolerance of an assembled PCR to a PCR inhibitor.

Compositions, kits and methods for synthesis and/or detection of nucleic acids

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San Jose, CA, United States of America

Ferrier Le