Cargill Inc.

Cj Cheiljedang Corporation

Genomatica, Inc.

Reg Life Sciences, LLC

Ls9, Inc.

Fernando A Sanchez-Riera

David B Weiner

Paula M Hicks

Sara C McFarlan

Brian J Brazeau

Christopher Solheid

Holly J Jessen

Steven John Gort

Wei Niu

Sherry R Kollmann

Mervyn Desouza

So-Young Kim

Young Wook Cho

Kwang Myung Cho

Jose Miguel Laplaza

Mervyn L De Souza

Yong-Uk Shin

Jin-Sook Chang

Hyewon Um

David Paul Weiner

Peter Luginbuhl

Stephen B Del Cardayre

Myong K Ko

Perry Y Liao

Simon Li

Toby Howard Richardson

Timothy Walter Abraham

Zhihao Hu

Douglas Conrad Cameron, Jr

William Schroeder

Lishan Zhao

Ellen Burke

Louis G Hom

Vikranth Arlagadda

Eli S Groban

John P Rosazza

Derek L Greenfield

Analia Bueno

Erin Kathleen Marasco

Joslin M Cuenca

Anil Bhagwan Khare

Mervyn L deSouza

Willy Obyn

Jean-Claude De Troostembergh

Joshua Lundorff

Brent Howard Hilbert

Monica Bhatia

Scott Allen Frykman

Kent Floy

Mary Jo Zidwick

Michael C M Cockrem

Sara C Mcfarlan

Sherry R Kollman

Jack Rosazza

Methods of producing fatty acid esters, such as fatty acid ethyl esters, from genetically engineered microorganisms are described.

Production of fatty acid esters

The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.

Methods and compositions for improved production of fatty acids and derivatives thereof

The disclosure relates to a novel glycerol purification process that produces partially refined waste glycerol for a variety of industrial applications. The disclosure encompasses a salt-containing partially refined glycerol composition that is suitable as a fermentation grade glycerol.

Production of partially refined waste glycerol

Described herein are microorganisms that produce methionine and related products from endogenous genes in a transsulfuration pathway, as well as from exogenous genes providing a direct sulfhydrylation pathway. Novel genes that are useful for methionine and SAMe production are disclosed.

Compositions and methods of producing methionine

Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

Polypeptides and biosynthetic pathways for the production of stereoisomers of monatin and their precursors

The invention provides for aminotransferase and oxidoreductase polypeptides and nucleic acids encoding such polypeptides. Also provided are methods of using such aminotransferase and oxidoreductase nucleic acids and polypeptides.

Aminotransferase and oxidoreductase nucleic acids and polypeptides and methods of using

There are provided bioproducts and methods of improving production of the bioproducts from engineered microbial cells, the methods comprising: providing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; and incubating the inoculated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. Also, provided are kits for practicing the methods of improving production of bioproducts.

Reduction of the toxic effect of impurities from raw materials by extractive fermentation

Methods and compositions that can be used to make monatin or salt thereof from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

Production of monatin and monatin precursors

A method is disclosed for increasing the specific activity of myo-inositol oxygenase. The method includes incubating a mixture including myo-inositol oxygenase and a non-sulfur containing reductant under conditions effective to increase the specific activity of the myo-inositol oxygenase. Also disclosed are methods for producing D-glucuronic acid and glucurono-&#x3b3;-lactone comprising incubating a mixture including myo-inositol, myo-inositol oxygenase, and oxygen under conditions effective to form 5 grams D-glucuronic acid per liter of the mixture to 400 grams D-glucuronic acid per liter of the mixture. Glucurono-&#x3b3;-lactone can be produced from the D-glucuronic acid product. Also disclosed are organisms and nucleic acids suitable for use in such methods.

Production of glucuronic acid using myo-inositol oxygenase from cryptococcus neoformans

Methods and systems for increasing the production of equilibrium reactions are described. In some embodiments, a method comprises removing enzymes from a reaction mixture once equilibrium is achieved, selectively re-adding enzymes and reactants for driving the product-forming reaction to product, and optionally recycling stabilized intermediates, by-products and/or co-products back into the reaction mixture. In some embodiments, a method comprises purifying the desired product from a reaction mixture and selectively recycling one or more components of the reaction mixture, such as original reactants, intermediates, co-products or by-products back into the reaction mixture as desired to improve titer. Systems for implementing the methods are also provided. In some embodiments the methods and systems are implemented for increasing the production of monatin and monatin derivatives, produced in multi-step equilibrium pathways.

Methods and systems for increasing production of equilibrium reactions

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Eden Prairie, MN, United States of America

Fernando A Sanchez-Riera