Maat Pharma

Institut National De La Recherche Agronomique

Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement

Fernanda Fonseca

Carole Schwintner

Hervé Affagard

Joël Dore

Catherine Juste

Patricia Lepage

Christel Maillet

Sylvie Rabot

Hervé Blottiere

Audrey Chapron

Olivier David

Herve Affagard

Joel Dore

The present invention relates to a method for preparing a fecal 5 microbiota sample of a donor subject. Said method includes the following steps: a) collecting at least one fecal microbiota sample from the donor subject; b) within a period of less than 5 minutes after collecting the sample, placing said sample obtained in step a) in an oxygen-tight collection device; c) mixing the sample obtained in step b) with at least one aqueous saline 10 solution containing at least one cryoprotectant and/or a filling agent; d) optionally, filtering the mixture obtained in step c), in particular by means of a filter comprising pores having a diameter of less than or equal to 0.7 mm, preferably less than or equal to 0.5 mm; and e) storing the mixture obtained in step c) or d) by freezing said mixture at a temperature 15 of between &#x2212;15&#xb0; C. and &#x2212;100&#xb0; C. Steps b) to e) are carried out in anaerobiosis.

Method of preparing a faecal microbiota sample

Method for lyophilisation of a simple of fecal microbiota. The present invention relates to a method for lyophilisation of a sample of fecal microbiota from a donor subject, comprising the following steps: A) mixing of a sample of fecal microbiota from a donor subject with a diluent selected from polyols, disaccharides to pentasaccharides, maltodextrins and mixtures thereof, and B) freezing the mixture obtained in A) at a temperature of less than &#x2212;50&#xb0; C., preferably of between &#x2212;70&#xb0; C. and &#x2212;100&#xb0; C., followed by the lyophilisation thereof.

Method of lyophilization of a sample of faecal microbiota

Growing community of inventors

Villepreux, France

Fernanda Fonseca