Boehringer Mannheim Gmbh

Boehringer Mannheim Corporaton

Fern DeLaCroix

Harvey Buck

Johann Berger

Juergen Schrenk

Hans Berger

Hans-Joachim Guder

The invention relates to an assay for determining an analyte in a fluid sample. Three receptors are used, with formation of a quaternary or four member complex. One of the receptors is bound to a solid phase, and binds to complexes of another receptor and the analyte. A third receptor is labelled, and it, too, binds analyte. The invention also involves the use of a displacement solution to wash fluid sample from solid phase after the first portion of the quaternary complex forms, i.e., prior to binding with the labelled receptor.

Method for sequential determination of an analyte in a fluid sample

An apparatus for determining an analyte in a sample, as well as a method for carrying this out, are disclosed. The apparatus utilizes a first zone containing a labeled substance, and a second zone which permits separation of a mobile detectable moiety from unreacted reaction component. Detectable moiety is the product of reaction between reaction component and labeled substance.

Apparatus for determining an analyte and method therefor

This invention relates to apparatus useful in determining a component or components of a test sample, as well as methods using these apparatus. Of particular interest are apparatus and methods which involve formation and determination of quarternary complexes.

Apparatus for determination of a component in a sample

The invention involves an apparatus useful in analysis of liquid samples. At least 3 zones are provided which contain reactants which interact with the analyte and each other, leading to the detection reaction. The apparatus also has a fluid application means for reception of a liquid, and a waste zone, which absorbs excess liquid after the detection reaction has taken place. The fluid application means and waste zone are positioned at opposite ends of the apparatus.

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Indianapolis, IN, United States of America

Fern DeLaCroix