Ab Kabi

Ferenc Merenyl

Nils Ake Jonsson

Lars-Erik Westlund

Disclosed are benzidine derivatives substituted in each of the 3,3'-positions by the substituent ##STR1## wherein W is hydrogen or an alkali metal, the subscript n is zero when A is the divalent branched chain alkylene having from 2 to about 7 carbon atoms, and n is one when A is straight chain divalent alkylene with 3 or 4 carbons. Also disclosed are the corresponding derivatives wherein the sulfonyl group is replaced by the carbonyl group. Further disclosed are chromogen-reactive indicator compositions having as their chromogen constituent one of the foregoing benzidine derivatives. These compositions (1) are useful to test for a substance for which there exists a specific oxygen-oxidoreductase, such as a sugar, an amino acid, uric acid, and the like, and thus (2) contain also a peroxidase, a hydrogen-peroxide-oxidoreductase, and a specific oxygen-oxidoreductase for the specific material in the testing of which the composition is to be used.

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Taby, Sweden

Ferenc Merenyl