Monsanto Technology LLC

Fenggao Dong

Byron Froman

Virginia Ursin

Steven E Screen

Thomas J LaRosa

Jennifer Simmons

Sam R Eathington

Stanton B Dotson

Nengbing Tao

Huachun Wang Larue

Frederic Achard

Edward James Cargill

Jonathan C Lamb

Zoe P McKiness

David V Butruille

Michael J Graham

Daniel J Ovadya

Richard J Lawrence

Ruth A Wagner

Charles L Armstrong

Brian M Hauge

Thomas J La Rosa

Yang Ju Im

Jennifer Gonzales

Thomas S Ream

Fengxing Du

Zoe McCuddin

Shengqiang Zhong

Pierre Sehabiague

Piyaraj Nan Newton

Brian W Gardunia

Daniel Challis

Christopher T Bauer

Christopher Oggero

Michael Graham

Stan Dotson

Fred Achard

Zoe Mccuddin

Provided are methods for increasing the efficiency of creating doubled haploid plants by increasing the number of chances of forming a double haploid seed through treatment of a monocot plant with a plant growth regulator. In certain embodiments, maize plants are produced that comprise multiple co-dominant ears. Also provided are plants comprising the potential to generate increased numbers of doubled haploid offspring.

Methods for creating doubled haploid plants

The invention provides novel methods to genotype haploid embryos using molecular assays. For example, quantitative methods for genotyping are provided. The methods provided also include providing a plurality of haploid kernels, determining the genotype of the haploid embryo of said kernels by distinguishing its genotype from the endosperm genotype, selecting a kernel having a desired genotype and producing doubled haploid plant from the selected kernel.

Methods for genotyping haploid embryos

Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

Methods and compositions for genome editing via haploid induction

The invention relates generally to methods and compositions concerning desaturase enzymes that modulate the number and location of double bonds in long chain poly-unsaturated fatty acids (LC-PUFA's). In particular, the invention relates to methods and compositions for improving omega-3 fatty acid profiles in plant products and parts using desaturase enzymes and nucleic acids encoding for such enzymes. In particular embodiments, the desaturase enzymes are&#x394;6-desaturases. Also provided are improved soybean oil compositions having SDA and a beneficial overall content of omega-3 fatty acids relative to omega-6 fatty acids.

Fatty acid desaturases from primula

Provided herein are delivery methods of a plant treatment agent to a tissue of a plant, wherein the tissue is contacted with a plant treatment agent and delivery is aided by a centrifugal force applied to the plant. Disclosed herein is the discovery that drastic improvements in treatment delivery occur when plants are centrifuged after being contacted, submerged, unsubmerged, soaked, etc. in a solution containing a plant treatment agent. In one embodiment, haploid plants contacted with a colchicine solution are centrifuged to reveal improved chromosomal doubling rates as compared to control plants.

Aided delivery of plant treatment agents

The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.

Methods for sequence-directed molecular breeding

The present invention provides methods for assembling DNA molecules in a predetermined order to produce a DNA construct useful inmediated transformation in plants. The method employs ligation independent cloning of separate DNA elements where one of the DNA elements contains T-DNA borders from. The invention further provides methods for assembling a construct containing an inverted repeat. Using this approach, DNA constructs are constructed rapidly, efficiently and directionally.

Methods for ligation independent cloning of DNA

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Chesterfield, MO, United States of America

Fenggao Dong