Other

Iowa State University Research Foundation Inc

City College of New York

The Research Foundation

Feng Liu

Robert R Alfano

Patrick S Schnable

Yan Fu

Ping Pei Ho

Wei Cai

Melvin Lax

Bidyut B Das

Samir M Ahmed

Gordon Emory Anderson

Leming M Wang

Kwong M Yoo

Quan-Zhen Wang

Xiangchun Liang

Yici Guo

Zhi-Wei Zang

Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5' or 3&#x2032; to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3&#x2032; of the ATG start codon and 5&#x2032; of the 3-frame His-tag DNA sequence.

Method of identifying an open reading frame using a nucleic acid molecule encoding multiple start codons and histidine tags

Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes, in each of the three possible reading frames, an ATG start codon and a histidine tag, and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences of interest 5&prime; or 3&prime; to the 3-frame His-tag DNA sequence. Vectors also are provided with cloning sites for inserting nucleotide sequences of interest 3&prime; of the ATG start codon and 5&prime; of the 3-frame His-tag DNA sequence.

Nucleic acid molecules encoding multiple start codons and histidine tags

Compositions and methods for identifying nucleotide fragments that contain an open reading frame are provided. Compositions comprise a nucleotide sequence that encodes histidine tags in each of the three possible reading frames and vectors comprising such a nucleotide sequence. The vectors may be provided with cloning sites for insertion of nucleotide sequences 5&prime; or 3&prime; to the 3-frame His-tag DNA sequence.

Nucleic acid molecules encoding histidine tags in three reading frames

A method and system for imaging a small object in or behind a highly scattering medium comprises a laser source for illuminating the object with an ultrashort collimated beam of light and a novel microscope for forming a magnified image of the object using light emergent from the highly scattering medium, the emergent light consisting of a scattered component and a non-scattered component. The novel microscope comprises an objective, an eyepiece and an aperture centered at the back focal plane of the objective. The aperture, which may be of a fixed or variable size and controlled electronically or by a computer serves to spatially filter the scattered light component of the light emergent from the highly scattering medium. The system may also comprise a streak camera or similar time resolving device positioned at the image plane of the microscope for temporally filtering the scattered light component of the light emergent from the highly scattering medium.

Microscope imaging inside highly scattering media

An apparatus utilizing non-linear optical signals for use in constructing a three-dimensional tomographic map of an in vivo biological tissue for medical disease detection purposes. In one embodiment, said apparatus comprises a stage for supporting the in vivo biological tissue; a laser for illuminating the in vivo biological tissue with a focused beam of laser light, the light emerging from the in vivo biological tissue comprising fundamental light, harmonic wave light, and fluorescence due to multi-photon excitation; a filter for selectively passing only at least one of the harmonic wave light and the fluorescence; one or more detectors for individually detecting each of the harmonic wave light and the fluorescence selectively passed; and a mechanism for moving the laser relative to the stage in x, y and z directions.

Non-linear optical tomography of turbid media

A technique for forming an image of an object in or behind a scattering medium. In one embodiment, the object is made luminescent, and the luminescent light is selected for imaging while the illuminating light is filtered out. The quality of the image can be further improved by selected the portion of the luminescence spectrum that is strongly absorbed by the scattering medium.

Technique for imaging an object in or behind a scattering medium

A method for imaging objects in highly scattering turbid media. According to one embodiment of the invention, the method involves using a plurality of intersecting source/detectors sets and time-resolving equipment to generate a plurality of time-resolved intensity curves for the diffusive component of light emergent from the medium. For each of the curves, the intensities at a plurality of times are then inputted into the following inverse reconstruction algorithm to form an image of the medium: ##EQU1## wherein W is a matrix relating output at source and detector positions r.sub.s and r.sub.d, at time t, to position r, .LAMBDA. is a regularization matrix, chosen for convenience to be diagonal, but selected in a way related to the ratio of the noise, &lt;nn&gt; to fluctuations in the absorption (or diffusion) X.sub.j that we are trying to determine:

Time-resolved diffusion tomographic 2D and 3D imaging in highly

A method for imaging objects in highly scattering turbid media. According to one embodiment of the invention, the method involves using a plurality of intersecting source/detectors sets and time-resolving equipment to generate a plurality of time-resolved intensity curves for the diffusive component of light emergent from the medium. For each of the curves, the intensities at a plurality of times are then inputted into the following inverse reconstruction algorithm to form an image of the medium:

Time-resolved diffusion tomographic imaging in highly scattering turbid

A system for imaging an object in or behind a highly scattering medium includes a light source for illuminating the highly scattering medium with a beam of light. The light emerging form the highly scattering medium consists of a ballistic component, snake-like component and a diffuse component. A 4F Fourier imaging system including a Fourier spatial filter located at 2F is used to form a time gated image of the emerging light, the time gated image consisting primarily of the ballistic component and the snake-like component.

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Bronx, NY, United States of America

Feng Liu