Nantong Licheng Biological Engineering Co. Ltd

Tianjin University

Feng Jin

Baohong Hou

Junbo Gong

Qiuxiang Yin

Jingkang Wang

Qinqing Gu

Yi Sun

Jiangbo Li

Kaifei Zhao

Shichao Du

Hongzhi Xia

Xiaohua Wu

Bo Tang

Yulei Zhu

Haiyan Xi

Shiping Tang

An ultrasonic-assisted method for preparing phosphatidylserine, comprising the following steps: adding 100-130 parts of phospholipid into a mixture of 150-200 parts of L-serine, 10-20 parts of anhydrous calcium chloride and 400-500 parts of pure water, adding 20-25 parts of phospholipase D for enzymatic hydrolysis reaction, and applying ultrasound in the enzymatic hydrolysis reaction for treatment. The present invention uses an ultrasonic treatment technology to assist phospholipase D to act on phosphatidylcholine and serine to undergo an enzymatic hydrolysis reaction to prepare phosphatidylserine, and at the same time, the ultrasonic frequency, ultrasonic intensity, ultrasonic power, ultrasonic time, ultrasonic temperature, enzyme activity and other parameters are controlled synergistically, so that the enzymatic hydrolysis conversion rate is 98% or higher.

Method for preparing phosphatidylserine by ultrasonic-assisted enzymatic hydrolysis

A method for preparing a new crystalline form of &#x3b3;-aminobutyric acid, including the steps of: S1: preparing a &#x3b3;-aminobutyric acid solution at an initial concentration of 0.5-1.0 g/mL by adding crude &#x3b3;-aminobutyric acid to water; adding an additive to the &#x3b3;-aminobutyric acid solution, raising the temperature to 50-80&#xb0; C., stirring to produce a clear solution; and S2: obtaining a suspension by evaporating water from the product of S1 under reduced pressure and at 50-80&#xb0; C.; obtaining a wet product by filtering the suspension; drying the wet product to obtain the new crystalline form of &#x3b3;-aminobutyric acid. The preparation method of the new crystalline form of &#x3b3;-aminobutyric acid is simple, easy to operate, low in energy consumption, economical and environmentally friendly. It is suitable for large-scale industrial production.

Crystalline form of gamma-aminobutyric acid and preparation method thereof

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Jiangsu, China

Feng Jin