Promega Corporation

Fen Huang

James Robert Hartnett

Trent Gu

John William Shultz

Dieter Klaubert

Wenhui Zhou

Michael R Slater

Paul Otto

Douglas R Storts

Katharine M Miller

Alexander Novikov

Elena Bolchakova

Galina A Velikodvorskaya

The present invention provides an assay for detection of oxidized glutathione (GSSG).

Oxidized glutathione assay

The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases.andpossess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.

Method of reverse transcription

The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species. The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms ofDNA polymerase in sequencing, reverse transcription, and amplification reactions.

[object Object]

The present invention provides compositions comprising thermostable DNA polymerases derived from hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterial species . The present invention also provides methods for utilizing naturally-occurring and non-naturally-occurring forms of DNA polymerase in sequencing, reverse transcription, and amplification reactions.

Thermophilic DNA polymerases from Thermoactinomyces vulgaris

The present invention relates to reverse transcription of RNA, and in particular to reverse transcription by thermostable DNA polymerases. and possess reverse transcriptase activity in the presence of magnesium or manganese ions. Methods, compositions, and kits for reverse transcription and RT-PCR are also provided.

The present invention provides methods and compositions for protein stabilization, particularly the stabilization of polymerases in aqueous solutions with cationic surfactants. The present invention further provides cationic surfactants, including polyethoxylated amines, that stabilize thermostable and thermolabile enzymes in solution. These surfactants stabilize the activity of various enzymes, including thermostable DNA polymerases, thermolabile DNA polymerases and reverse transcriptases.

Polymerase stabilization by polyethoxylated amine surfactants

The present invention relates to compositions of thermostable DNA polymerases derived from the hyperthermophilic eubacteria. In particular, the present invention comprises thermostable DNA polymerases from the hyperthermophilic eubacterium known as Thermotoga neapolitana. The present invention provides methods for utilizing naturally-occurring and non-naturally-occurring forms of T. neopolitana DNA polymerase. The T. neopolitana DNA polymerases of the present invention are used in combination with other compounds, including but not limited to pyrophosphatase and DNA polymerases from other thermophilic or hyperthermophilic organisms.

Thermophilic DNA polymerases from Thermotoga neapolitana

The present invention relates to thermostable DNA polymerases derived from the hyperthermophilic eubacteria, and Thermotoga neapolitana in particular. The present invention provides means for isolating and producing the enzymes from these thermostable DNA polymerases, which are useful in many recombinant DNA techniques, especially such techniques as thermal cycle sequencing and nucleic acid amplification.

Growing community of inventors

Madison, WI, United States of America

Fen Huang