Koninklijke Philips Corporation N.v.

Minicare B.v.

Siemens Healthineers Nederland B.v.

Concateno UK Limited

Koninklijke Philips Electronics N.v.

Cozart Bioscience Limited

Van Lankvelt, Petrus J. W.

Femke Karina De Theije

Johannes Wilhelmus Weekamp

Sergei Shulepov

Albert Hendrik Jan Immink

Jeroen Hans Nieuwenhuis

Eduard Gerard Marie Pelssers

Thea Van Der Wijk

Coen Adrianus Verschuren

Alexander Marc Van Der Lee

Johannes Joseph Hubertina Barbara Schleipen

Dominique Maria Bruls

Petrus Johannes Wilhelmus Van Lankvelt

Ahmed Mohammed Taki Jehanli

Wendy Uyen Dittmer

Gordon Thomas Jowett

Bernardus Jozef Maria Beerling

Wilhelmina Maria Hardeman

Wendela Meertens

Mara Johanna Jacoba Sijbers

Albert H J Immink

Joost Lambert Max Vissers

Sytske Foppen

Willemina Maria Huijnen-Keur

Danielle Walthera Maria Kemper-Van De Wiel

Roland Antonius Johannes Gerardus Smits

Sandra Marlin

Hendrik Jan De Graaf

Petrus J W Van Lankvelt

Jeroen H Niewenhuis

Ahmed M T Jehanli

Eduard G M Pelssers

Gordon T Jowett

André Albert Hendrik Jan Immink

Godefridus J Verhoeckx

Hendrik S Van Damme

Wendy U Dittmer

Harold J A Brans

Mara J J Sijbers

The invention relates to a method for the detection of target components that comprise label particles, for example magnetic particles (). The method includes (a) collecting the target components at a binding surface () of a carrier (); (b) directing an input light beam (L, L, L) into the carrier such that it is totally internally reflected in an investigation region () at the binding surface (); and (c) determining the amount of light of an output light beam (L, L, L) that comprises at least some of the totally internally reflected light. Evanescent light generated during the total internal reflection is affected (absorbed, scattered) by target components and/or label particles () at the binding surface () and will therefore be missing in the output light beam (L). This can be used to determine the amount of target components at the binding surface () from the amount of light in the output light beam (L, L, L). A magnetic field generator () is optionally used to generate a magnetic field (B) at the binding surface () by which magnetic label particles () can be manipulated, for example attracted or repelled.

Method of detecting label particles

The present invention relates to a method for detection of at least one target molecule in a blood sample comprising at least the following steps: (a) providing a blood sample; (b1) contacting said sample with an antioxidant and applying said sample to a filter to separate the blood cells from said sample; and/or (b2) applying said sample to a filter to separate the blood cells from said sample, wherein said filter comprises an antioxidant; (c) contacting said sample with a first binding molecule in the cartridge, wherein said first binding molecule is attached to a magnetic particle, and wherein said first binding molecule is capable of specifically binding to said at least one target molecule; (d) contacting said sample with a second binding molecule, wherein said second binding molecule is attached to a sensor surface, and wherein said second binding molecule is capable of specifically binding to said at least one target molecule; and (e) detecting the magnetic particles on the sensor surface. The present invention also relates to the use of an antioxidant in the process of detection of at least one target molecule in a blood sample as well as to cartridges for insertion into an assay device for detection of at least one target molecule in a blood sample.

Use of antioxidants in methods and means for detection of target molecules in a blood sample

The invention relates to a method and a device for the detection of magnetic particles () in a sample chamber (). After introduction of the sample into said sample chamber (), the magnetic particles () are first retained within the sample chamber () and kept away from the sensing surface () by an appropriate magnetic field (B) to allow for an incubation of the sample with reagents. A reference measurement may be made during this incubation period (T), preferably at the end thereof. After incubation, the magnetic particles () are allowed to contact the sensing surface () where a target measurement can be conducted.

Magnetic particle detection with incubation period

A cartridge for detection of target components in a liquid sample includes a sample chamber, at least two reservoirs that can be furnished with magnetic particles, and at least two corresponding sensitive zones in which solved magnetic particles and/or target components can be detected. When a magnetic actuation field of a given configuration is established in the sample chamber, the magnetic particles of different reservoirs migrate predominantly to different sensitive zones. Thus a mixing of magnetic particles can be avoided.

Cartridge for assays with magnetic particles

A system and method wherein components of a reagent such as labeled antibodies are separated from a biologically active sensor surface by depositing the reagent on a carrier surface distinct from a sensor surface in a detection region. The present device provides a short, well-defined and controlled, pre-incubation time between the particles of interest in the sample fluid and the reagent, thereby increasing the reproducibility by providing all components in one detection region such as a detection chamber.

Methods and systems for detecting

A microelectronic sensor device for the detection of target components with label or magnetic particles includes a carrier with a binding surface at which target components can collect and optionally bind to specific capture elements. An input light beam is transmitted into the carrier and totally internally reflected at the binding surface. The amount of light in the output light beam is detected by a light detector. Evanescent light generated during the total internal reflection is affected by target components and/or label particles at the binding surface and will be missing in the output light beam. This is used to determine the amount of target components at the binding surface from the amount of light in the output light beam. A magnetic field generator is optionally used to generate a magnetic field at the binding surface by which magnetic label particles can be manipulated, such as attracted or repelled.

Microelectronic sensor device for detecting label particles

The invention provides a device for detecting drugs of abuse or other compounds in saliva. The invention thus provides a device for detecting the presence of one or more analytes in a saliva sample, comprising: (a) One or more pre-treatment regions for specifically or non-specifically removing at least a part of the fraction of the saliva sample interfering with detection of the one or more analytes; and (b) A detection region comprising a biosensor surface, the surface comprising: molecules capable of specifically binding the one or more analytes; or the one or more analytes and/or analyte analogues.

Device and methods for detecting analytes in saliva

A sensor device () for detecting the presence of a target molecule () in a sample, is disclosed. The sensor device comprises a measurement sensor () comprising a first moiety () for forming a binding couple with a first further moiety comprising the target molecule () and a detectable label () and a reference sensor () comprising a second moiety () for forming a further binding couple with a second further moiety comprising a further detectable label ('). The sensor device is adapted to generate a first detection signal () from the detection of the detectable label () in the first further moiety bound to the first moiety () and to generate a second detection signal (&#x2032;) from the detection of the further detectable label (&#x2032;) in the second further moiety bound to the second moiety (), wherein at least during operation of the sensor device the second further moiety is expected to be present in a predefined amount such that the value of the second detection signal (&#x2032;) falls within an expected signal value window when the binding reaction of the second further moiety to the second moiety takes place as expected. An apparatus comprising such a sensor device, methods of operating the sensor device and apparatus and a sample for use with the sensor device are also disclosed.

Magnetic sensor device, method of operating such a device and sample

The invention provides a device for detecting drugs of abuse or other compounds in saliva. The invention thus provides a device for detecting the presence of one or more analytes in a saliva sample, comprising: (a) One or more pre-treatment regions for specifically or non-specifically removing at least a part of the fraction of the saliva sample interfering with detection of the one or more analytes; and (b) A detection region comprising a biosensor surface, the surface comprising: molecules capable of specifically binding the one or more analytes; or the one or more analytes and/or analyte analogs.

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Berghem, Netherlands

Femke Karina De Theije