The Methodist Hospital

Wellsim Biomedical Technologies, Inc

Liu, Fei

The Methodist Hospital Dba Houston Methodist

Hu, Ye

Fan, Jia

Sun, Dali

Liang, Kai

Fei Liu

Ye Hu

Yuchao Chen

Kai Liang

Dali Sun

Jia Fan

A rapid, ultrasensitive and inexpensive nanoplasmon-enhanced scattering (nPES) assay that directly quantifies tumor-derived EVs from as little as 1 μ{umlaut over ({acute over (ι)})}, of plasma is described herein. This assay uses binding of gold nanospheres and nanorods with EV- and tumor-derived EV-specificities to produce a local plasmon effect that enhances tumor-derived EV detection sensitivity and specificity. This nPES approach is also a non-invasive method for assessing pancreatic cancer stage and treatment response that can be easily refined for clinical use, and is readily adapted for diagnosis and monitoring of other conditions with disease-specific EV proteins.

Nanoplasmonic quantification of tumor-derived extracellular vesicles in plasma microsamples for detection and treatment monitoring

An isolation chip for separating and isolating target particles from a bioliquid sample includes a reagent reservoir, a first filtration membrane, a second filtration membrane, a first chamber, and a second chamber. The reagent reservoir includes a first sidewall and a second sidewall opposite to the first sidewall. The first filtration membrane is disposed at an upper portion of the first sidewall. The reagent reservoir defines a first window at a lower portion of the first sidewall. The second filtration membrane is disposed at the second sidewall. The first chamber is connected to the reagent reservoir through the first filtration membrane. The second chamber is connected to the reagent reservoir through the second filtration membrane. A device and a method for detecting the target particles are further provided.

Isolation chip for isolating target particles from liquid sample, and device and method for detecting the target particles

Growing community of inventors

Cupertino, CA, United States of America

Fei Liu