Snu Rdb Foundation

Jiangsu Academy of Agricultural Sciences

Guotai (taizhou) Center of Technology Innovation for Veterinary Biologicals, Taizhou

Fei Hao

Lei Zhang

Qiyan Xiong

Zhixin Feng

Yanfei Yu

Daesub Song

Jongwoo Lim

Guoqing Shao

Beibei Liu

Yuan Gan

Rong Chen

Yun Bai

Yongjie Liu

Xing Xie

Long Zhao

Minjoo Yeom

Lulu Xu

Wenliang Li

A viral vector delivery system for both respiratory and digestive tracts of pigs and an application thereof are provided. The viral vector delivery system includes a backbone plasmid and a helper plasmid. The backbone plasmid is produced by inserting a full-length cDNA of porcine enterovirus B (PEVB) into a pUC57 plasmid. The helper plasmid is produced by inserting a green fluorescent protein-coding gene into a plasmid pCAG-T7-polymerase. The viral vector delivery system can be constructed with high efficiency, can quickly cause a cytopathic effect (CPE) after infecting cells, has a viral titer up to 10TCID/mL, and can maintain high stability. A pathogenic epitope for the respiratory and digestive tracts of pigs is inserted into the backbone plasmid to produce a vaccine antigen, which is non-pathogenic, has high stability and a high viral titer, and allows a prominent immunization effect after being inoculated in pigs.

Viral vector delivery system for both respiratory and digestive tracts of pigs and application thereof

Growing community of inventors

Nanjing, China

Fei Hao