Rhode Island Council on Postsecondary Education, Statutory Sucessor To the Rhode Island Board of Education, and Rhode Island Board of Governors for Higher Education

The Board of Governors for Higher Education

Fatemeh Akhlaghi

Mwlod Ghareeb

Anisha E Mendonza

Disclosed is a method of measuring tacrolimus levels in a subject. In exemplary embodiments, the method comprises the steps of: collecting oral fluid from the subject; homogenizing the oral fluid; combining the homogenized oral fluid with a precipitating solvent; separating oral fluid components on a liquid chromatography column by gradient elution with a mixture of a solvent A and a solvent B, wherein the solvent A is about 2 mM ammonium acetate/0.1% (v/v) formic acid in water and solvent B is about 2 mM ammonium acetate/0.1% (v/v) formic acid in MeOH and wherein the amount of solvent B is increased from about 50% (v/v) to about 98% (v/v); and quantifying the amount of tacrolimus in the oral fluid by mass spectrometry.

Growing community of inventors

Wakefield, RI, United States of America

Fatemeh Akhlaghi