Other

Vital Biosciences, Inc.

Kazemzadeh, Farnoud

Wong, Alexander Sheung Lai

Farnoud Kazemzadeh

Alexander Sheung Lai Wong

Alexander Wong

Iman Khodadad

Shahid Abbas Haider

Iman Khodadadzadeh

Alexander Sheung Wong

Methods and devices for analyzing a sample from a subject are provided. A device includes one or more coherent light sources and one or more partially coherent light sources. The light sources are configured to emit first and second wavelengths of electromagnetic radiation towards an imaging chamber configured to hold blood cells. The light sources are aligned to illuminate a single common area of the imaging chamber. The device includes optics positioned to receive light from the imaging chamber, and a detector in optical communication with the optics. The detector is configured to detect fluorescence emission and backscatter from blood cells when present in the imaging chamber. The device includes mixers/unmixers configured to replicate and mix first and second signals representative of the responses to the first and second wavelengths after interacting with the plurality of blood cells to generate spectro-spatial responses.

Device, method, and system for sample analysis

A system may include emitters configured to emit radiation at a first wavelength of electromagnetic (EM) radiation and a second wavelength of EM radiation towards a biological tissue, and receivers configured to receive responses to the first and second wavelengths of EM radiation after the wavelengths of EM radiation interact with the biological tissue. The system may also include a signal mixer unit configured to perform operations that include replicate and mix first signals representative of the responses to the first wavelength of EM radiation received by the receivers and second signals representative of the responses to the second wavelength of EM radiation received by the receivers to generate a set of spectro-spatial responses, replicate and mix the spectro-spatial responses to generate markers, and replicate and mix the markers and user-selected markers to output a sequence associated with characterization of the biological tissue.

Imaging biological tissue or other subjects

There is disclosed a system and method for measuring the polarization of light which utilizes a spatially-varying polarization beam. This measurement is analyzed and improved using novel imaging methods and image processing methods on a digital signal processing (DSP) system to determine the polarization of light. Various embodiments are described which can measure light polarization, determine polarizing material distributions, determine the optical rotary dispersion of samples, and determine the circular dichroism of samples. In certain embodiments, the binding constants and binding activity of molecular samples can be achieved. Given its reduced complexity and size, the system may be configured to be portable such that the system may be used in various applications outside of a lab setting.

System, method and apparatus for measuring polarization using spatially-varying polarization beams

A method may include emitting a band of electromagnetic (EM) radiation towards a specimen that covers at least a first and a second wavelength of EM radiation. The method may also include receiving, at a first receiver configured to receive the first wavelength of EM radiation, responses to the EM radiation after the EM radiation interacts with the specimen; and receiving, at a second receiver configured to receive the second wavelength of EM radiation, responses to the EM radiation after the EM radiation interacts with the specimen. The method may also include extracting markers from a combination of first signals representative of the received responses at the first receiver and second signals representative of the received responses at the second receiver, the extracting including replicating and mixing the first signals and the second signals to extract the plurality of markers.

Electromagnetic wave emittance-based specimen analysis

There is disclosed a novel system and method for multiview, multispectral, polarimetric, light-field, and high dynamic range imaging in a concurrent manner specifically capturing information at different spectral bands and light polarizations simultaneously. The present system and method is capable of () concurrent imaging of multiple spectral bands (including spectral bands beyond the visible region of the electromagnetic spectrum), proportional or greater than the number of filters used in the device, () concurrent imaging of multiple light polarizations (Stokes vectors), () acquiring images at different point-of-view of the same scene and/or object that allow for topographical reconstruction, () concurrent imaging of the multiple depth of fields that allow for light-field imaging, and () concurrent imaging of multiple simulated exposures of the detector that allow for high dynamic range imaging, all at the same time using a single sensor in the same imaging system enclosure.

Imaging system and method for concurrent multiview multispectral polarimetric light-field high dynamic range imaging

There is disclosed a novel system and method for achieving ultra-resolution, ultra-wide field-of-view multispectral and hyperspectral holographic microscopy and quantitative phase contrast microscopy. In an embodiment, the method comprises: providing a stationary illumination source; acquiring a plurality of low-resolution holograms of an image subject from different locations utilizing a subpixel sensor-scanning synthetic aperture mechanism whereby a detector scanning translationally, radially and/or rotationally; processing the acquired holograms utilizing a processing algorithm corresponding to the scanning motion of the detector used to acquire the holograms; and reconstructing a subpixel ultra-resolution image of the image subject based on the processed holograms; whereby, a desired synthetic aperture is achieved without loss of resolution. The multispectral and hyperspectral aspect is achieved in the novel system and method by use of different combination of illumination sources (i.e., LEDs, laser sources, broadband lamps, etc.) and wavelength selection mechanisms (i.e., bandpass spectral filters, acousto-optical and liquid crystal tunable filters, a dispersing element, etc.).

System, method and apparatus for ultra-resolved ultra-wide field-of-view multispectral and hyperspectral holographic microscopy

There is disclosed a novel system and method for improving spectral resolution and signal-to-noise ratio of a captured spectrum by employing digital beam reforming, digital slicing, and digital multiplexing. In an embodiment, the method comprises: and entrance aperture for the light to enter the apparatus, a collimating element (refractive or reflective), a dispersive element (single- or multi-axis), a focusing element (refractive or reflective), and a linear or array detector for acquisition of the spectrum. The spectrum is then digitally beam reformed, digitally beam sliced to increase spectral resolution, and digitally beam multiplexed to increase the signal-to-noise ratio. In addition the disclosed invention is also capable of performing digital beam refocusing which means the optical focusing power can be chosen such that its performance surpasses the performance of any analog optical focusing element thereby further increasing the spectral resolution and the signal-to-noise ratio of the spectrum. The invention can be used as a stand-alone system or can be used in conjunction of any spectrometer even those augmented with optical slicer technologies. The degree of improvement to the spectral resolution is completely tunable and has no bearing of the efficiency of the instrument.

System, method and apparatus for improving the spectral resolution and signal-to-noise ratio of optical spectrometer using digital beam refocusing, reforming, slicing, and multiplexing

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Waterloo, Canada

Farnoud Kazemzadeh