Illumina Corporation

Farnaz Absalan

Gordon M Cann

Tarun Kumar Khurana

Yir-Shyuan Wu

Jeffrey S Fisher

Arash Jamshidi

Yan-you Lin

Sarah Stuart

Frank J Steemers

Jeffrey G Mandell

Alex Aravanis

Yan-You Lin

Dmitry K Pokholok

Leila Bazargan

Steven Norberg

Dimitry K Pokholok

In accordance with embodiments herein a method for capturing cells of interest in a digital microfluidic system is provided, comprising utilizing a droplet actuator to transport a sample droplet to a microwell device. The microwell device includes a substrate having a plurality of microwells that open onto a droplet operations surface of the microwell device. The sample droplet includes cells of interest that enter the microwells. The method introduces capture beads to the microwells, and the capture elements are immobilized on the capture beads. The method utilizes the droplet actuator to transport a cell lysis reagent droplet to the microwell device. Portions of the cell lysis reagent droplet enter the microwells and, during an incubation period, cause the cells of interest to release analyte that is captured by the capture elements on the capture beads.

Digital microfluidic system for single-cell isolation and characterization of analytes

A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

Polynucleotide enrichment using CRISPR-Cas systems

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Farnaz Absalan