Cubist Pharmaceuticals, Incorporated

Fariba Houman

Xiaoyu Shen

Jianshi Tao

Paul L Gallant

Mandana Sassanfar

Paul R Schimmel

Janice E Kranz

Christoph Kaufmann

Nianjun Tao

Yan Qiu

The present invention relates to isolated and/or recombinant nucleic acids which encode Candida cytoplasmic tryptophanyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida cytoplasmic tryptophanyl-tRNA synthetase or portion thereof. Also disclosed are constructs comprising the nucleic acids of the present invention, host cells comprising a recombinant nucleic acid or construct, and methods of producing a Candida cytoplasmic tryptophanyl-tRNA synthetase, portion thereof, or fusion protein comprising the same. Also described are tester strains, which are cells engineered to rely on the function of a Candida cytoplasmic tryptophanyl-tRNA synthetase or functional fragment thereof encoded by an introduced cloned gene, and which can be used in a method of detecting an inhibitor of Candida cytoplasmic tryptophanyl-tRNA synthetase function. The invention further relates to isolated and/or recombinant Candida cytoplasmic tryptophanyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida cytoplasmic tryptophanyl-tRNA synthetase or portion thereof, methods of use of these polypeptides in an assay to identify inhibitors of Candida cytoplasmic tryptophanyl-tRNA synthetase function, and antibodies reactive with Candida cytoplasmic tryptophanyl-tRNA synthetases.

Candida cytoplasmic tryptophanyl-tRNA synthetase proteins, nucleic acids and strains comprising same

The present invention relates to isolated and/or recombinant nucleic acids which encode Candida isoleucyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida isoleucyl-tRNA synthetase or portion thereof. Also disclosed are constructs comprising the nucleic acids of the present invention, host cells comprising a recombinant nucleic acid or construct, and methods of producing a Candida isoleucyl-tRNA synthetase, portion thereof, or fusion protein comprising same. Also described are tester strains, which are cells engineered to rely on the function of a Candida isoleucyl-tRNA synthetase or functional fragment thereof encoded by an introduced cloned gene, and which can be used in a method of detecting an inhibitor of Candida isoleucyl-tRNA synthetase function. The invention further relates to isolated and/or recombinant Candida isoleucyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida isoleucyl-tRNA synthetase or portion thereof, methods of use of these polypeptides in an assay to identify inhibitors of Candida isoleucyl-tRNA synthetase function, and antibodies reactive with Candida isoleucyl-tRNA synthetases.

Candida isoleucyl-tRNA synthetase proteins, nucleic acids and strains

The present invention relates to isolated and/or recombinant nucleic acids which encode Candida tyrosyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida tyrosyl-tRNA synthetase or portion thereof. Also disclosed are constructs comprising the nucleic acids of the present invention, host cells comprising a recombinant nucleic acid or construct, and methods of producing a Candida tyrosyl-tRNA synthetase, portion thereof, or fusion protein comprising same. Also described are tester strains, which are cells engineered to rely on the function of a Candida tyrosyl-tRNA synthetase or functional fragment thereof encoded by an introduced cloned gene, and which can be used in a method of detecting an inhibitor of Candida tyrosyl-tRNA synthetase function. The invention further relates to isolated and/or recombinant Candida tyrosyl-tRNA synthetases, portions thereof, or fusion proteins comprising a Candida tyrosyl-tRNA synthetase or portion thereof, methods of use of these polypeptides in an assay to identify inhibitors of Candida tyrosyl-tRNA synthetase function, and antibodies reactive with Candida tyrosyl-tRNA synthetases.

Candida tyrosyl-tRNA synthetase proteins, nucleic acids and strains

Recombinant nucleic acids which encode aminoacyl-tRNA sythetases of helicobacter origin have been isolated. These nucleic acids can be used to make expression constructs and transformed host cells for the production of helicobacter aminoacyl-tRNA synthetases. They can also be used in the further isolation of nucleic acids related by DNA sequence similarities, which also encode helicobacter aminoacyl-tRNA synthetases, or portions thereof. A further embodiment of the invention is antisense nucleic acid which can hybridize to the nucleic acid which encodes the aminoacyl-tRNA synthetase of helicobacter. The invention also relates to isolated and/or recombinant helicobacter aminoacyl-tRNA synthetases. Antibodies which bind to these enzymes can be made and can be used in the purification and study of the enzymes. Also described are tester strains, which are cells engineered to rely on the function of the tRNA synthetase encoded by an introduced cloned gene, and which can be used to test the effectiveness of drug candidates in the inhibition of an essential aminoacyl-tRNA synthetase encoded by an introduced cloned helicobacter gene.

Growing community of inventors

Belmont, MA, United States of America

Fariba Houman