Shanghai Jiao Tong University

Shanghai Longyao Biotechnology Inc., Ltd.

Fanlin Li

Xuanming Yang

Yangxin Fu

Xin Wang

Shengqin Ye

Huihui Zhang

A chimeric antigen receptor (CAR) that includes a targeting moiety, a transmembrane domain, a co-stimulatory domain and an intracellular signaling domain, wherein the targeting moiety specifically binds to variable regions Vα and Vβ of a T cell receptor (TCR) and/or fragments thereof.

Chimeric antigen receptor and use thereof

Provided by the present invention is a chimeric antigen receptor comprising a co-stimulatory receptor, the chimeric antigen receptor having a structure of scFv(X)-(Y)CD3zeta-2A-(Z); X comprises a tumortargeting antibody or a ligand or receptor capable of specifically binding to a tumor; Y is an intracellular region of the co-stimulatory receptor, and Z is a co-stimulatory receptor that is selected from among ICOS, CD28, CD27, HVEM, LIGHT, CD40L, 4-1BB, OX40, DR3, GITR, CD30, TIMI, SLAM, CD2, CD226. Further provided by the present invention are CAR-T cells that are constructed by means of a recombinant expression vector of the described chimeric antigen receptor, a preparation method therefor and an application thereof. The CAR-T cells described in the present invention significantly improve the tumor-killing abilities and amplification abilities thereof.

Chimeric antigen receptor comprising co-stimulatory receptor and application thereof

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Shanghai, China

Fanlin Li