Mgi Tech Co., Ltd.

Tencent Technology (shenzhen) Company Limited

Byd Company Limited

Genemind Biosciences Company Limited

Bgi Genomics Co., Limited

Century Technology (shenzhen) Corporation Limited

Bgi Shenzhen

Shenzhen Yuanrunxin Electronics Co., Ltd.

Bgi Health Service Co., Ltd.

Fang Zhou Chen

Hui Fu Jiang

Ya Gao

Lin Yang

Yanyan Zhang

Lei Li

Jing Guo

Xun Xu

Qin Yan

Feng Bo Jiang

Xiuqing Zhang

Peng Zeng

Yuqian Wang

Luyang Zhao

Shengpei Chen

Yuxiang Chen

Jie Jian

Guodong Huang

Haojun Jiang

Jun Wang

Li Zhang

Jian Wang

Jun Ming Yan

Bin Hua Liu

Cheng Chen

Tao Yang

Fan Fan

Bin Liu

Huanming Yang

Shida Zhu

Cheng Wu

Zheng Chen

Yuanlian Huang

Weiwei Xie

Haiping Zhang

Ling Zhou

Guowu Huang

Jia Zhao

Huifei Chen

Xianghua Chai

Sen Lu

Yuying Yuan

Shuai Shen

Shaohong Zhang

Yan-Juan Zhang

Yifan Xie

Zhi-Rong Xie

Yicong Wang

Na Zhong

Xuchao Li

Tongming Wu

Xuehan Zhuang

Xiaoyu Pan

Jialou Zhu

Fuman Jiang

The present invention relates to a nucleoside analog having the structure of the following formula, a method for preparing the nucleoside analog, and an application of the nucleoside analog in nucleic acid sequencing, etc.

Nucleoside analogue, preparation method and application

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises a first primer and a second primer, wherein the first primer comprises a first specific sequence, a first random sequence, and a first universal sequence, the first specific sequence is located at the 3' end of the first primer, the first random sequence is located at the 5′ end of the first primer, and the first universal sequence is located between the first specific sequence and the first random sequence; the second primer comprises a second specific sequence, a second random sequence, and a second universal sequence, the second specific sequence is located at the 3′ end of the second primer, the second random sequence is located at the 5′ end of the second primer, and the second universal sequence is located between the second specific sequence and the second random sequence, wherein the first random sequence and the second random sequence are inversely complementary.

PCR primer pair and application thereof

Lamp

Provided is a PCR primer pair, comprising: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on 3' end of the first primer, and the first random sequence is located on 5&#x2032; end of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on 3&#x2032; end of the second primer, and the second random sequence is located on 5&#x2032; end of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence respectively, and the first random sequence and the second random sequence are inverse complementary.

Provided are a PCR primer pair and an application thereof. The PCR primer pair comprises: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on end 3' of the first primer, and the first random sequence is located on end 5&#x2032; of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on end 3&#x2032; of the second primer, and the second random sequence is located on end 5&#x2032; of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence, respectively; the first random sequence and the second random sequence are reverse complementary; a predetermined restriction enzyme cutting site is connected between the first specific sequence and the first random sequence; and a predetermined restriction enzyme cutting site is connected between the second specific sequence and the second random sequence.

Nucleoside or nucleotide analog compounds having the structure shown below, a method for preparing them, and applications in nucleic acid sequencing are disclosed. The compounds have formula (I): 

Disclosed are a method and a system for determining genome copy number variation, which relates to the technical field of bioinformatics. The method and the system have clinical feasibility, and can precisely detect a micro-deletion/micro-duplication area of 0.5 M under the situation of using data of about 50 M.

Method and system for determining copy number variation

Provided is a method for isolating exosomal DNA, the method comprising: (i) providing a sample, wherein the sample comprises a blood sample from peripheral blood of a pregnant woman; and (ii) subjecting the sample to isolating, thus obtaining the exosomal DNA. Also provided are a method for detecting a blood sample, a method for constructing a sequencing library on a blood sample, a method for high-throughput sequencing an exosomal DNA sequencing library, a method for non-invasive prenatal gene detection, a device for non-invasive prenatal gene detection, and a kit for detecting a blood sample and use thereof.

Method for non-invasive prenatal diagnosis based on exosomal DNA and application thereof

A shift register which allows narrower framing of a display screen includes cascade-connected shift register modules. Each shift register module is electrically coupled to first and second scan lines and outputs scanning signals to the first and the second scan lines in a time-division manner in response to first to fourth clock signals. Each shift register module includes an initial circuit, a reset circuit, a first output circuit, and a second output circuit. The initial circuit receives a trigger signal for activating the shift register module. The initial circuit controls the first output circuit to output a signal to the first scan line based on the first clock signal and controls the second output circuit to output a signal to the second scan line according to the second clock signal.

Shift register allowing narrower bezel and display apparatus based thereon

A method and apparatus for defending against a DNS attack includes receiving a DNS request from a request source and obtaining an IP address of the request source, calculating a characteristic value of the IP address, and searching an internal memory for preset identifier information corresponding to the characteristic value. Based on the preset identifier information corresponding to the characteristic value being a detection identifier indicating an uncertainty of an existence of an attack risk in the DNS request, the method includes obtaining request record information and an unblocking time corresponding to the characteristic value, the request record information including a number of request times from the request source in a predetermined period. Based on the number of request times exceeding a preset request threshold, determining that the DNS request is an attack request and discarding the DNS request.

Method and apparatus for defending against DNS attack, and storage medium

A data synchronization method, system and apparatus are provided. The method includes receiving a request including data to be uploaded, from a client, and responding to the request after data is successfully obtained from the client, and storing the obtained data. For data whose size is less than a threshold value, a synchronization request is sent to a standby server to request the standby server to store the data. Otherwise a second type work log, including information indicating that data that has not been synchronized, is generated and stored. Data whose size is greater than or equal to the threshold value is not synchronized immediately, but is recorded in the work log. In some cases, data whose size is less than the threshold value, but fails to be synchronized, is recorded in the work log. Synchronization of this data may be subsequently completed according to the work log, so that the synchronization of this data can avoid affecting synchronization of other data. A first-type work log comprising information about the data may be generated and stored in response to the data being synchronized to the standby server successfully. Data that has not been synchronized to the standby server, according to the indication information, may be locally read and the data sent to the standby server to be stored. Data may be sent to the standby server to replace other data, in response to verification codes, such as hash codes, being inconsistent. The first and second work logs may also be sent to a log backup device. Responding to the uploading request and sending data to the standby server may occur asynchronously.

Data synchronization method, system, and apparatus using a work log for synchronizing data greater than a threshold value

The present invention provides a method for determining the nucleic acid composition in a total nucleic acid mixture comprising a first nucleic acid and a second nucleic acid. The method comprises: 1) treating the total nucleic acid mixture with a bisulfate, to convert the non-methylated cytosine in the total nucleic acid mixture into uracil, and obtain a converted total nucleic acid mixture; 2) subjecting the converted total nucleic acid mixture to multiplexed fluorescent quantitative PCR using a first set of amplification primers and a second set of amplification primers; and 3) based on the ratio R of the methylated amplification product to the non-methylated amplification product of the predetermined nucleic acid fragment, a methylation proportion Mof the predetermined nucleic acid fragment in the first nucleic acid, and a methylation proportion Mof the predetermined nucleic acid fragment in the second nucleic acid, determining the nucleic acid composition in the total nucleic acid mixture.

Method for determining nucleic acid composition of nucleic acid mixture

The current invention is directed to methods for noninvasive detection of fetal genetic abnormalities by large-scale sequencing of nucleotides from maternal biological sample. Further provided are methods to remove GC bias from the sequencing results according to the difference in GC content of a chromosome. The current invention not only makes the detection much more accurate but also represents a comprehensive method for fetal aneuploidy detection including sex chromosome disorders such as XO, XXX, XXY, and XYY, etc.

Method for determining fetal chromosomal abnormality

This invention discloses a charging/discharging protective circuit for a secondary battery pack, having an over-charging/discharging voltage comparator, a reference voltage source and a battery status decision circuit. There is also a sampling circuit having a sequential pulse generator for generating pulses for selecting one of the batteries in the battery pack for testing purposes. The pulse generator provides M-channel gating pulses to the selection circuit of the battery under test and provides sampling pulses to the over-charging/discharging voltage comparators. The reference voltage source has a regulated output circuit. This invention uses time division inspection methods to provide a cost-effective solution for inspecting batteries in a battery pack.

Protective circuits for secondary battery packs

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Shenzhen, China

Fang Zhou Chen