Lawrence Livermore National Security, LLC

Fang Qian

Eric B Duoss

Christopher M Spadaccini

Cheng Zhu

Sarah Elyse Baker

Joshua R Deotte

Elizabeth K Wheeler

Anna Nikolaevna Ivanovskaya

Kris Kulp

Marcus A Worsley

Joshua D Kuntz

Satinderpall Singh Pannu

Angela C Tooker

Vanessa M Tolosa

Jennifer Marie Knipe

Nikola Dudukovic

Thomas Yong Han

Samantha Ruelas

Chao Huang

Theodore F Baumann

Kedar G Shah

Joshuah K Stolaroff

Robin R Miles

Mihail Bora

Du Nguyen

Heeral J Sheth

Tayyab Ishaq Suratwala

Yinmin Wang

Harry B Radousky

James T Cahill

Jinkyu Han

Tammy Olson

Tyler Martin Fears

Margaret Windy McNerney

Rebecca Dylla-Spears

Koroush Sasan

Erika Jo Fong

Jonathan Tesner Davis

Haiyin Chen

Bryan D Hudson

Timothy Dexter Yee

Hawi Bacha Gemeda

Karen Ruth Dubbin

Dylan Dahlquist

Cameron David Meyers

Joel Destino

Pui Ching Lan

Bio-ink comprising freeze-dried cells, methods of making a living structure from a bio-ink material of freeze-dried cells, and methods of using the living structure for biosensing, tissue regeneration, environment sensing, drug discovery, catalysis, and/or clinical implementation are described herein.

Bio-ink structures and methods of producing the same

According to one embodiment, a method includes forming a structure by printing an ink, the ink including a glass-forming material, and heat treating the formed structure for converting the glass-forming material to glass. According to another embodiment, an ink composition includes a glass-forming material and a solvent.

Engineered feedstocks for additive manufacture of glass

The present disclosure relates to an engineered, additively manufactured, microfluidic cellular structure formed from a plurality of cells, wherein the cells are each formed from a plurality of interconnected elements. The cells have voids and each cell is open at upper ends thereof. The cells each communicate at a point below its upper end with a common channel. The cells are each configured to accept a fluid and operate to channel the fluid into the common channel and to hold the fluid received therein for later selective withdrawal from the structure.

Systems and methods for reaction and transport engineering via cellular fluidics

A method includes simultaneously measuring electrophysiological responses and contractility responses of a plurality of cells forming a tissue culture using a system comprising a sensor array configured to simultaneously measure the electrophysiological responses and the contractility responses of the plurality of cells forming the tissue culture. Fabrication techniques for making such systems include: forming a sensor array comprising an interpenetrating arrangement of IDEs and electrodes of a MEA in or on a substrate surface; forming a plurality of contacts for interfacing the system with one or more external devices in or on the substrate surface; and forming leads between the plurality of contacts and the sensor array.

Sensor array and apparatus for simultaneous observation of tissue electrophysiology, contractility, and growth

In accordance with one aspect of the presently disclosed inventive concepts, a metal aerogel includes a plurality of metal nanowires formed into a porous three-dimensional structure, where pores in the structure are anisotropic.

Ultralight conductive metallic aerogels

An engineered unit cell is disclosed for flowing a fluid therethrough in three dimensions. The unit cell may have a substrate with a plurality of flow channels around and between struts formed within the substrate. The struts may each be formed with a desired shape and orientation within the substrate to achieve a desired degree of fluid flow through the flow channels, in each of one of three dimensions, through the unit cell.

Lattice microfluidics

A sensor array for simultaneously measuring electrophysiological responses and contractility responses of a tissue includes: a substrate; a multi-electrode array (MEA) disposed in or on the substrate; and a plurality of interdigitized electrodes (IDEs) disposed in or on the substrate. The MEA and the IDEs are interpenetrating within a plane substantially parallel to an upper surface of the substrate. Systems for measuring such responses using the sensor array may also include contacts and/or connectors for interfacing with external control devices, electrodes, and cell culture components such as a chamber and lid. Fabrication techniques for making such systems include: forming a sensor array comprising an interpenetrating arrangement of IDEs and electrodes of a MEA in or on a substrate surface; forming a plurality of contacts for interfacing the system with one or more external devices in or on the substrate surface; and forming leads between the plurality of contacts and the sensor array.

In one embodiment, a system includes a bioreactor coupled to a substrate. The bioreactor includes: a plurality of walls defining a reservoir; a plurality of fluidic channels in at least some of the walls; a fluidic inlet in fluidic communication with the reservoir via, the fluidic channels; a fluidic outlet in fluidic communication with the reservoir via the fluidic channels; and one or more sensors coupled to the reservoir, each sensor being configured to detect one or more of: environmental conditions in the reservoir; and physiological conditions of one or more cells optionally present in the reservoir. In another embodiment, a method includes delivering media to a reservoir of a bioreactor; delivering a plurality of cells to the reservoir, controlling a reservoir temperature and a reservoir gas composition; using one or more of the sensors to monitor environmental and physiological conditions; and reporting the environmental and/or physiological conditions.

Miniaturized, automated in-vitro tissue bioreactor

The present invention relates to surface roughening methods and more particularly to a method for electrochemical roughening of thin film macro- and micro-electrodes. In one embodiment, an electrochemical etch template is formed comprising polymer particles adsorbed on a surface of a substrate to be roughened, followed by electrochemically etching of exposed regions of the substrate between the polymer particles in the electrochemical etch template so as to selectively roughen the surface of the substrate. In another embodiment, a surface of the electrode is immersed in either a adsorbing acidic solution, such as sulfuric acid, or a non-adsorbing acidic solution, such as perchloric acid, followed by electrochemically pulse etching the surface of the substrate at a narrow frequency range for adsorbing acidic solutions, or at a wide frequency range for non-adsorbing acidic solutions.

Method for electrochemical roughening of thin film electrodes

A system is disclosed for harvesting at least one of mechanical or thermal energy. The system may have a flexible substrate, a plurality of electrically conductive nanowires secured to the substrate, and a plurality of electrically conductive metal layers. The metal layers may be disposed on the substrate and spaced apart from one another along a length of the substrate. The metal layers may be in electrically conductive contact with various ones of the nanowires. At least two of the metal layers may be attachable to an external device. At least one of movement or flexing of the substrate produces an output voltage across the metal layers.

Harvesting mechanical and thermal energy by combining nanowires and phase change materials

Disclosed here is a three-dimensional electronic scaffold, comprising a porous scaffold and a plurality of micro-strain gauges distributed spatially inside the porous scaffold, wherein the micro-strain gauges are adapted to detect contraction force. Also disclosed is a method comprising detecting and mapping intra-tissue cardiac contraction force of one or more cardiac cells or tissues disposed in a three-dimensional electronic scaffold, wherein the three-dimensional electronic scaffold comprises a porous scaffold and a plurality of micro-strain gauges distributed spatially inside the porous scaffold and in contact with the cardiac cells or tissues, and wherein the micro-strain gauges are adapted to detect contraction force of the cardiac cells or tissues.

Three-dimensional electronic scaffold for cardiac applications

In one embodiment, a process includes creating a mixture of an aqueous component, nanowires and nanoparticles, and a hydrophobic solvent and allowing migration of the nanowires to the hydrophobic solvent, where the nanoparticles remain in the aqueous component. Moreover, the nanowires and nanoparticles are in the aqueous component before the migration.

Cross-phase separation of nanowires and nanoparticles

Growing community of inventors

Santa Cruz, CA, United States of America

Fang Qian