Corning Incorporated

Fang Lai

Ye Fang

Brian L Webb

Laurent A G Picard

John Christopher Mauro

Joydeep Lahiri

Yulong Hong

Valerie Claudine Lacarriere

Srikanth Raghavan

Alexander Mikhailovich Efremov

Mingqian He

Thomas Mark Leslie

Alain R E Carre

Helen Samson

Alain Re Carre

Susan Marie Gasper

Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins.

Method and device for protein delivery into cells

The invention relates to G protein-coupled receptor (GPCR) microarrays on porous substrates for structural or functional analyses of GPCRs, and methods of preparing porous substrate surfaces for receiving membranes that comprise GPCRs. In one embodiment, a GPCR microarray of the invention comprises a membrane adhered to an upper surface of a porous substrate, the membrane spanning across a plurality of pores on the porous substrate to form a plurality of cavities having sufficient geometry to permit entry of assay reagents into each cavity, thereby allowing access of assay reagents to both sides of GPCR in the membrane.

Membrane arrays and methods of manufacture

Fluorescent dyes useful for detecting a target material in biological assays an electron donating moiety which is linked by a conjugated &#x3c0;-electron bridge to an electron accepting moiety. The &#x3c0;-electron bridge includes at least one carbocyclic ring structure or heterocyclic ring structure. The electron accepting moiety is a carboxylic acid, a salt of a carboxylic acid, or has the formula:  wherein: Xis selected from the group consisting of H, CH, and (CH)-Z, where Zis a monovalent anion; and nis an integer from 1 through 10; and Qis O or S.

Fluorescent dyes

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Painted Post, NY, United States of America

Fang Lai