Illumina Corporation

Illumina Cambridge Limited

Fan Zhang

Frank J Steemers

Dmitry K Pokholok

Steven Norberg

Ramesh Ramji

Lena Christiansen

Kevin L Gunderson

Molly Min He

Niall Anthony Gormley

Amirali Kia

Igor Yu Goryshin

Jason Richard Betley

Eric Hans Vermaas

Rigo Pantoja

Natalie Morrell

Wouter Meuleman

Rosamond Jackson

Gareth Jenkins

Avgousta Ioannou

Jacqueline Weir

Steven J Norberg

Jerushah Thomas

Weihua Chang

Megan Chu

Patrizia Iavicoli

Jeffrey Brodin

Igor Goryshin

The invention relates to a system and methods for enhancing access to nuclear informational molecules, such as DNA, RNA, and proteins, by analytical biomolecules, such as transposome complexes, by treating nuclei with a nuclear permeability enhancer, and to methods of using nuclear membrane, cell membrane, and external compartmentalization approaches as contiguity preserving elements.

Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments

Embodiments of systems, methods, and compositions provided herein relate to methods of simultaneously analyzing multiple analytes in a single sample using a single assay. Some embodiments relate to simultaneous analysis of DNA and RNA in a single sample, for example, to the simultaneous generation of DNA and RNA libraries.

Analysis of multiple analytes using a single assay

Systems, methods, and compositions provided herein relate to preparation of beads encapsulating biomolecules for performing sequential reactions on the biomolecules. Some embodiments include preparation of nucleic acid reactions within the bead, wherein the bead includes pores that allow diffusion of molecules into or out of the beads while retaining other molecules of interest.

Modulating polymer beads for DNA processing

Embodiments provided herein relate to methods and compositions for preparing an immobilized library of barcoded DNA fragments of a target nucleic acid, identifying genomic variants, determining the contiguity information, phasing information, and methylation status of the target nucleic acid.

Contiguity preserving transposition

Embodiments of systems, methods, and compositions provided herein relate to hollow beads encapsulating single cells. Some embodiments include performing multiple co-assays on a single cell encapsulated within a hollow bead, including nucleic acid sequencing, preparing nucleic acid libraries, determining methylation status, identifying genomic variants, or protein analysis.

Contiguity particle formation and methods of use

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San Diego, CA, United States of America

Fan Zhang